Preparation of stable Pd nanocubes and their use in biological labeling

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00381927" target="_blank" >RIV/60077344:_____/12:00381927 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389013:_____/12:00381927 RIV/68378050:_____/12:00381927

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.colsurfb.2012.05.023" target="_blank" >http://dx.doi.org/10.1016/j.colsurfb.2012.05.023</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.colsurfb.2012.05.023" target="_blank" >10.1016/j.colsurfb.2012.05.023</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Preparation of stable Pd nanocubes and their use in biological labeling

Popis výsledku v původním jazyce

Stable Pd nanocubes (PdNC) with the average size 15 nm were prepared by the controlled reduction of sodium tetrachloropalladate with ascorbic acid in water, in the presence of polyvinylpyrrolidone and potassium bromide. Morphology of the particles was characterized by transmission electron microscopy (TEM) and their stability in the colloidal solution was verified by dynamic light scattering (DLS). It has been demonstrated that the Pd nanocubes can be distinguished from commercial Au nanospheres in a standard TEM microscope by means of automated image analysis. In the next step, the PdNC were successfully conjugated to immunoglobulin proteins and used for the detection of a specific protein (nucleophosmin) on ultrathin sections of HeLa cells. Our experiments showed that PdNC can be used for multiple immunolabeling in combination with commercial Au nanospheres.

Název v anglickém jazyce

Preparation of stable Pd nanocubes and their use in biological labeling

Popis výsledku anglicky

Stable Pd nanocubes (PdNC) with the average size 15 nm were prepared by the controlled reduction of sodium tetrachloropalladate with ascorbic acid in water, in the presence of polyvinylpyrrolidone and potassium bromide. Morphology of the particles was characterized by transmission electron microscopy (TEM) and their stability in the colloidal solution was verified by dynamic light scattering (DLS). It has been demonstrated that the Pd nanocubes can be distinguished from commercial Au nanospheres in a standard TEM microscope by means of automated image analysis. In the next step, the PdNC were successfully conjugated to immunoglobulin proteins and used for the detection of a specific protein (nucleophosmin) on ultrathin sections of HeLa cells. Our experiments showed that PdNC can be used for multiple immunolabeling in combination with commercial Au nanospheres.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Colloids and Surfaces B-Biointerfaces

ISSN

0927-7765

e-ISSN

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Svazek periodika

100

Číslo periodika v rámci svazku

1 December

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

205-208

Kód UT WoS článku

000307683900029

EID výsledku v databázi Scopus

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