Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00367418" target="_blank" >RIV/60077344:_____/11:00367418 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/11:43883012 RIV/61389013:_____/11:00367418

Výsledek na webu

<a href="http://dx.doi.org/10.1017/S1431927611000547" target="_blank" >http://dx.doi.org/10.1017/S1431927611000547</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S1431927611000547" target="_blank" >10.1017/S1431927611000547</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy

Popis výsledku v původním jazyce

The application of palladium nanoparticles as electron-dense markers for labeling in both transmission and scanning electron microscopy requires their conjugation to a specific protein. The conjugation protocol described here includes the dihydrolipoic acid capping of Pd nanoparticles and their subsequent covalent attachment to protein molecules such as streptavidin, protein A, or avidin. The single-step reaction was mediated using the cross-linking agent ethyl-3-(3- dimethylaminopropyl)carbodiimide. The final Pd conjugates were fully functional, as demonstrated by labeling of ultrathin resin sections of either bovine serum albumin or secretory granules of the salivary gland isolated from the partially fed female Ixodes ricinus tick. The results were quantified, evaluated, and compared with results obtained using commercially available gold particle conjugates. Both the streptavidin-Pd conjugate and commercial streptavidin-Au conjugate were used to detect fucosylated glycans.

Název v anglickém jazyce

Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy

Popis výsledku anglicky

The application of palladium nanoparticles as electron-dense markers for labeling in both transmission and scanning electron microscopy requires their conjugation to a specific protein. The conjugation protocol described here includes the dihydrolipoic acid capping of Pd nanoparticles and their subsequent covalent attachment to protein molecules such as streptavidin, protein A, or avidin. The single-step reaction was mediated using the cross-linking agent ethyl-3-(3- dimethylaminopropyl)carbodiimide. The final Pd conjugates were fully functional, as demonstrated by labeling of ultrathin resin sections of either bovine serum albumin or secretory granules of the salivary gland isolated from the partially fed female Ixodes ricinus tick. The results were quantified, evaluated, and compared with results obtained using commercially available gold particle conjugates. Both the streptavidin-Pd conjugate and commercial streptavidin-Au conjugate were used to detect fucosylated glycans.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microscopy and Microanalysis

ISSN

1431-9276

e-ISSN

—

Svazek periodika

17

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

810-816

Kód UT WoS článku

000295609100020

EID výsledku v databázi Scopus

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