Microbiological ecology and association of Bacillus thuringiensis in chicken feces originating from feed

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00383105" target="_blank" >RIV/60077344:_____/12:00383105 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/content/pdf/10.1007%2Fs00284-012-0231-3" target="_blank" >http://link.springer.com/content/pdf/10.1007%2Fs00284-012-0231-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00284-012-0231-3" target="_blank" >10.1007/s00284-012-0231-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Microbiological ecology and association of Bacillus thuringiensis in chicken feces originating from feed

Popis výsledku v původním jazyce

To explain the association of Bacillus thuringiensis (Bt) with animal feces, an ecological analysis in chickens was conducted by introducing a cry- strain marked by production of green fluorescent protein (GFP). After feeding with the tagged Bt strains,the feces of the tested chickens were collected at different times, isolated, and the morphology of Bt was observed. It was shown that Bt strain HD-73GFP in spore form could be isolated from feces of chickens for a period of 13 d, and then it disappearedthereafter. Bt could be detected only up to day 4 (but not thereafter), when chickens were fed with vegetative cells of HD-73GFP. To confirm the source of newly isolated strains, the gfp gene was examined by polymerase chain reaction (PCR), which showedthat all the isolated strains harbored the marker gene. Recent data from isolation and PCR had suggested that fecal Bt strains had originated from food.

Název v anglickém jazyce

Microbiological ecology and association of Bacillus thuringiensis in chicken feces originating from feed

Popis výsledku anglicky

To explain the association of Bacillus thuringiensis (Bt) with animal feces, an ecological analysis in chickens was conducted by introducing a cry- strain marked by production of green fluorescent protein (GFP). After feeding with the tagged Bt strains,the feces of the tested chickens were collected at different times, isolated, and the morphology of Bt was observed. It was shown that Bt strain HD-73GFP in spore form could be isolated from feces of chickens for a period of 13 d, and then it disappearedthereafter. Bt could be detected only up to day 4 (but not thereafter), when chickens were fed with vegetative cells of HD-73GFP. To confirm the source of newly isolated strains, the gfp gene was examined by polymerase chain reaction (PCR), which showedthat all the isolated strains harbored the marker gene. Recent data from isolation and PCR had suggested that fecal Bt strains had originated from food.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EH - Ekologie – společenstva

OECD FORD obor

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Projekt

<a href="/cs/project/2B08003" target="_blank" >2B08003: Změny biodiversity komárů - vektorů patogenních agens, v souvislosti se změnami klimatu.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Current Microbiology

ISSN

0343-8651

e-ISSN

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Svazek periodika

65

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

784-791

Kód UT WoS článku

000310073500021

EID výsledku v databázi Scopus

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