The colonization of Bacillus thuringiensis strains in bryophytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00471433" target="_blank" >RIV/60077344:_____/17:00471433 - isvavai.cz</a>

Výsledek na webu

<a href="http://journals.tubitak.gov.tr/biology/issues/biy-17-41-1/biy-41-1-5-1510-16.pdf" target="_blank" >http://journals.tubitak.gov.tr/biology/issues/biy-17-41-1/biy-41-1-5-1510-16.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3906/biy-1510-16" target="_blank" >10.3906/biy-1510-16</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The colonization of Bacillus thuringiensis strains in bryophytes

Popis výsledku v původním jazyce

In our previous study, several Bacillus thuringiensis (Bt) strains were isolated from bryophyte samples, indicating that bryophytes could serve as Bt reservoirs in the wild. SFR13 is a wild strain isolated from the bryophyta Physcomitrium japonicum. In order to understand its ecological properties, green fluorescent protein (GFP)-labelled SFR13 (SFR13GFP) was generated to evaluate the colonization capability in bryophytes, using dynamic tracing and cell counting to observe the process and patterns of colonization. Our results showed that genetic stability, growth curve dynamics, and insecticidal crystal production were not affected by GFP expression in Bt. Fluorescence microscopy was used to track the dynamic distribution of SFR13GFP. Distribution patterns showed that SFR13GFP can establish stable and long-term colonization in leaves and stems by the 26th day after inoculation. A better understanding of how Bt colonizes plants in the wild will not only result in increased knowledge of plant-microbe interactions but will also lead to a more successful and reliable use of bacterial inoculants.

Název v anglickém jazyce

The colonization of Bacillus thuringiensis strains in bryophytes

Popis výsledku anglicky

In our previous study, several Bacillus thuringiensis (Bt) strains were isolated from bryophyte samples, indicating that bryophytes could serve as Bt reservoirs in the wild. SFR13 is a wild strain isolated from the bryophyta Physcomitrium japonicum. In order to understand its ecological properties, green fluorescent protein (GFP)-labelled SFR13 (SFR13GFP) was generated to evaluate the colonization capability in bryophytes, using dynamic tracing and cell counting to observe the process and patterns of colonization. Our results showed that genetic stability, growth curve dynamics, and insecticidal crystal production were not affected by GFP expression in Bt. Fluorescence microscopy was used to track the dynamic distribution of SFR13GFP. Distribution patterns showed that SFR13GFP can establish stable and long-term colonization in leaves and stems by the 26th day after inoculation. A better understanding of how Bt colonizes plants in the wild will not only result in increased knowledge of plant-microbe interactions but will also lead to a more successful and reliable use of bacterial inoculants.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Turkish Journal of Biology

ISSN

1300-0152

e-ISSN

—

Svazek periodika

41

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

TR - Turecká republika

Počet stran výsledku

8

Strana od-do

41-48

Kód UT WoS článku

000394539800005

EID výsledku v databázi Scopus

2-s2.0-85013436348