Cantaloupe Facilitates Salmonella Typhimurium Survival Within and Transmission Among Adult House Flies (Musca domestica L.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43918242" target="_blank" >RIV/62156489:43210/21:43918242 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16810/20:43878436 RIV/62157124:16810/21:43878436

Výsledek na webu

<a href="https://doi.org/10.1089/fpd.2020.2818" target="_blank" >https://doi.org/10.1089/fpd.2020.2818</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1089/fpd.2020.2818" target="_blank" >10.1089/fpd.2020.2818</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cantaloupe Facilitates Salmonella Typhimurium Survival Within and Transmission Among Adult House Flies (Musca domestica L.)

Popis výsledku v původním jazyce

Salmonella entericaserovar Typhimurium is a pathogen harbored by livestock and shed in their feces, which serves as an acquisition source for adult house flies. This study used a green fluorescent protein (GFP) expressing strain ofSalmonellaTyphimurium to assess its acquisition by and survival within house flies, and transmission from and between flies in the presence or absence of cantaloupe. Female house flies were exposed to manure inoculated with either sterile phosphate-buffered saline or GFP-SalmonellaTyphimurium for 12 h, then used in four experiments each performed over 24 h. Experiment 1 assessed the survival of GFP-SalmonellaTyphimurium within inoculated flies. Experiment 2 determined transmission of GFP-SalmonellaTyphimurium from inoculated flies to cantaloupe. Experiment 3 assessed fly acquisition of GFP-SalmonellaTyphimurium from inoculated cantaloupe. Experiment 4 evaluated transmission of GFP-SalmonellaTyphimurium between inoculated flies and uninoculated flies in the presence and absence of cantaloupe. GFP-SalmonellaTyphimurium survived in inoculated flies but bacterial abundance decreased between 0 and 6 h without cantaloupe present and between 0 and 6 h and 6 and 24 h with cantaloupe present. Uninoculated flies acquired GFP-SalmonellaTyphimurium from inoculated cantaloupe and bacterial abundance increased in cantaloupe and flies from 6 to 24 h. More uninoculated flies exposed to inoculated flies acquired GFP-SalmonellaTyphimurium when cantaloupe was present than when absent. We infer that the presence of a shared food source facilitated the transfer of GFP-SalmonellaTyphimurium from inoculated to uninoculated flies. Our study demonstrated that house flies acquired, harbored, and excreted viable GFP-SalmonellaTyphimurium and transferred bacteria to food and each other. Understanding the dynamics of bacterial acquisition and transmission of bacteria between flies and food helps in assessing the risk flies pose to food safety and human health.

Název v anglickém jazyce

Cantaloupe Facilitates Salmonella Typhimurium Survival Within and Transmission Among Adult House Flies (Musca domestica L.)

Popis výsledku anglicky

Salmonella entericaserovar Typhimurium is a pathogen harbored by livestock and shed in their feces, which serves as an acquisition source for adult house flies. This study used a green fluorescent protein (GFP) expressing strain ofSalmonellaTyphimurium to assess its acquisition by and survival within house flies, and transmission from and between flies in the presence or absence of cantaloupe. Female house flies were exposed to manure inoculated with either sterile phosphate-buffered saline or GFP-SalmonellaTyphimurium for 12 h, then used in four experiments each performed over 24 h. Experiment 1 assessed the survival of GFP-SalmonellaTyphimurium within inoculated flies. Experiment 2 determined transmission of GFP-SalmonellaTyphimurium from inoculated flies to cantaloupe. Experiment 3 assessed fly acquisition of GFP-SalmonellaTyphimurium from inoculated cantaloupe. Experiment 4 evaluated transmission of GFP-SalmonellaTyphimurium between inoculated flies and uninoculated flies in the presence and absence of cantaloupe. GFP-SalmonellaTyphimurium survived in inoculated flies but bacterial abundance decreased between 0 and 6 h without cantaloupe present and between 0 and 6 h and 6 and 24 h with cantaloupe present. Uninoculated flies acquired GFP-SalmonellaTyphimurium from inoculated cantaloupe and bacterial abundance increased in cantaloupe and flies from 6 to 24 h. More uninoculated flies exposed to inoculated flies acquired GFP-SalmonellaTyphimurium when cantaloupe was present than when absent. We infer that the presence of a shared food source facilitated the transfer of GFP-SalmonellaTyphimurium from inoculated to uninoculated flies. Our study demonstrated that house flies acquired, harbored, and excreted viable GFP-SalmonellaTyphimurium and transferred bacteria to food and each other. Understanding the dynamics of bacterial acquisition and transmission of bacteria between flies and food helps in assessing the risk flies pose to food safety and human health.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Foodborne Pathogens and Disease

ISSN

1535-3141

e-ISSN

—

Svazek periodika

18

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

49-55

Kód UT WoS článku

000558370800001

EID výsledku v databázi Scopus

2-s2.0-85099342265