Environmental and Sex Effects on Bacterial Carriage by Adult House Flies (Musca domestica L.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43918089" target="_blank" >RIV/62156489:43210/20:43918089 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16810/20:43878437

Výsledek na webu

<a href="https://doi.org/10.3390/insects11070401" target="_blank" >https://doi.org/10.3390/insects11070401</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/insects11070401" target="_blank" >10.3390/insects11070401</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Environmental and Sex Effects on Bacterial Carriage by Adult House Flies (Musca domestica L.)

Popis výsledku v původním jazyce

Adult house flies frequent microbe-rich sites such as urban dumpsters and animal facilities, and encounter and ingest bacteria during feeding and reproductive activities. Due to unique nutritional and reproductive needs, male and female flies demonstrate different interactions with microbe-rich substrates and therefore dissemination potential. We investigated culturable aerobic bacteria and coliform abundance in male and female flies (n = 107) collected from urban (restaurant dumpsters) and agricultural (dairy farm) sites. Whole-fly homogenate was aerobically cultured and enumerated on nonselective (tryptic soy agar; culturable bacteria) and selective (violetred bile agar, VRBA; coliforms) media. Unique morphotypes from VRBA cultures of agricultural flies were identified and tested for susceptibility to 14 antimicrobials. Female flies harbored more bacteria than males and there was a sex by site interaction with sex effects on bacterial abundance at the urban site. Coliform abundance did not differ by sex, site or sex within site. Both male and female flies carried antimicrobial-resistant (AMR) bacteria: 36/38 isolates (95%) were resistant to GREATER-THAN OR EQUAL TO1 antimicrobial, 33/38 were multidrug-resistant (GREATER-THAN OR EQUAL TO2), and 24/38 isolates were resistant to GREATER-THAN OR EQUAL TO4 antimicrobials. Our results emphasize the role of house flies in harboring bacteria including AMR strains that pose a risk to human and animal health.

Název v anglickém jazyce

Environmental and Sex Effects on Bacterial Carriage by Adult House Flies (Musca domestica L.)

Popis výsledku anglicky

Adult house flies frequent microbe-rich sites such as urban dumpsters and animal facilities, and encounter and ingest bacteria during feeding and reproductive activities. Due to unique nutritional and reproductive needs, male and female flies demonstrate different interactions with microbe-rich substrates and therefore dissemination potential. We investigated culturable aerobic bacteria and coliform abundance in male and female flies (n = 107) collected from urban (restaurant dumpsters) and agricultural (dairy farm) sites. Whole-fly homogenate was aerobically cultured and enumerated on nonselective (tryptic soy agar; culturable bacteria) and selective (violetred bile agar, VRBA; coliforms) media. Unique morphotypes from VRBA cultures of agricultural flies were identified and tested for susceptibility to 14 antimicrobials. Female flies harbored more bacteria than males and there was a sex by site interaction with sex effects on bacterial abundance at the urban site. Coliform abundance did not differ by sex, site or sex within site. Both male and female flies carried antimicrobial-resistant (AMR) bacteria: 36/38 isolates (95%) were resistant to GREATER-THAN OR EQUAL TO1 antimicrobial, 33/38 were multidrug-resistant (GREATER-THAN OR EQUAL TO2), and 24/38 isolates were resistant to GREATER-THAN OR EQUAL TO4 antimicrobials. Our results emphasize the role of house flies in harboring bacteria including AMR strains that pose a risk to human and animal health.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10616 - Entomology

Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Insects

ISSN

2075-4450

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

12

Strana od-do

401

Kód UT WoS článku

000557822800001

EID výsledku v databázi Scopus

2-s2.0-85087122099