Five functional adipokinetic peptides expressed in the corpus cardiacum of the moth genus Hippotion (Lepidoptera, Sphingidae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00391874" target="_blank" >RIV/60077344:_____/13:00391874 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0167011513000670" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0167011513000670</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.regpep.2013.03.029" target="_blank" >10.1016/j.regpep.2013.03.029</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Five functional adipokinetic peptides expressed in the corpus cardiacum of the moth genus Hippotion (Lepidoptera, Sphingidae)

Popis výsledku v původním jazyce

This is the first study that finds five adipokinetic hormones (AKHs) in the corpus cardiacum of an insect. From two species of the sphingid moth genus Hippotion, eson and celerio, three novel and two known AKHs were isolated and sequenced: Hipes-AKH-I) and its Thr7 analogue (Hipes-AKH-II); two nonapeptides are pGlu-Leu-Thr-Phe-Thr-Ser-Ser-Trp-Gly amide (Manse-AKH) and its Thr7 analogue (Hipes-AKH-III), as well as a decapeptide pGlu-Leu-Thr-Phe-Ser-Ser-Gly-Trp-Gly-Gln amide (Manse-AKH-II). All sequenceswere confirmed by identical behaviour of natural and synthetic peptides in liquid chromatography coupled to electrospray tandem mass spectrometry. Conspecific injections of all five peptides in synthetic form and low doses caused hyperlipaemia in H. eson. Our results suggest that five genes code for the mature peptides, which are very likely released during flight to provide energy for long distance migration in this genus via lipid oximation. As all five peptides are active at low doses

Název v anglickém jazyce

Five functional adipokinetic peptides expressed in the corpus cardiacum of the moth genus Hippotion (Lepidoptera, Sphingidae)

Popis výsledku anglicky

This is the first study that finds five adipokinetic hormones (AKHs) in the corpus cardiacum of an insect. From two species of the sphingid moth genus Hippotion, eson and celerio, three novel and two known AKHs were isolated and sequenced: Hipes-AKH-I) and its Thr7 analogue (Hipes-AKH-II); two nonapeptides are pGlu-Leu-Thr-Phe-Thr-Ser-Ser-Trp-Gly amide (Manse-AKH) and its Thr7 analogue (Hipes-AKH-III), as well as a decapeptide pGlu-Leu-Thr-Phe-Ser-Ser-Gly-Trp-Gly-Gln amide (Manse-AKH-II). All sequenceswere confirmed by identical behaviour of natural and synthetic peptides in liquid chromatography coupled to electrospray tandem mass spectrometry. Conspecific injections of all five peptides in synthetic form and low doses caused hyperlipaemia in H. eson. Our results suggest that five genes code for the mature peptides, which are very likely released during flight to provide energy for long distance migration in this genus via lipid oximation. As all five peptides are active at low doses

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/NT11513" target="_blank" >NT11513: Profilování neurosteroidních konjugátů a dalších kyselých metabolitů v tělních tekutinách a jejich perspektiva v klinické diagnostice</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Regulatory Peptides

ISSN

0167-0115

e-ISSN

—

Svazek periodika

184

Číslo periodika v rámci svazku

JUN 10

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

85-95

Kód UT WoS článku

000320220700013

EID výsledku v databázi Scopus

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