Two novel tyrosine-containing peptides (Tyr4) of the adipokinetic hormone family in beetles of the families Coccinellidae and Silphidae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00450536" target="_blank" >RIV/60077344:_____/15:00450536 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/article/10.1007%2Fs00726-015-2011-4" target="_blank" >http://link.springer.com/article/10.1007%2Fs00726-015-2011-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00726-015-2011-4" target="_blank" >10.1007/s00726-015-2011-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Two novel tyrosine-containing peptides (Tyr4) of the adipokinetic hormone family in beetles of the families Coccinellidae and Silphidae

Popis výsledku v původním jazyce

Novel members of the adipokinetic hormone family have been identified from the corpora cardiaca (CC) of two species of beetles representing two families, the Silphidae and the Coccinellidae. A crude CC of the burying beetle, Nicrophorus vespilloides wasactive in mobilizing trehalose in a heterologous assay using the cockroach Periplaneta americana, whereas the CC extract of the ladybird beetle, Harmonia axyridis, exhibited no hypertrehalosemic activity. Primary sequences were elucidated by LC-MS/MS revealing an octapeptide with an unusual tyrosine residue at position 4 for each species: pGlu-Leu-Thr-Tyr-Ser-Thr-Gly-Trp amide for N. vespilloides (code-named Nicve-AKH) and pGlu-Ile-Asn-Tyr-Ser-Thr-Gly-Trp amide for H. axyridis (code-named Harax-AKH). Moreover, synthetic peptides were shown to be active in the heterologous cockroach assay system, but Harax-AKH only at a dose of 30 pmol. It appears that the tyrosine residue at position 4 can be used as a diagnostic feature for certain bee

Název v anglickém jazyce

Two novel tyrosine-containing peptides (Tyr4) of the adipokinetic hormone family in beetles of the families Coccinellidae and Silphidae

Popis výsledku anglicky

Novel members of the adipokinetic hormone family have been identified from the corpora cardiaca (CC) of two species of beetles representing two families, the Silphidae and the Coccinellidae. A crude CC of the burying beetle, Nicrophorus vespilloides wasactive in mobilizing trehalose in a heterologous assay using the cockroach Periplaneta americana, whereas the CC extract of the ladybird beetle, Harmonia axyridis, exhibited no hypertrehalosemic activity. Primary sequences were elucidated by LC-MS/MS revealing an octapeptide with an unusual tyrosine residue at position 4 for each species: pGlu-Leu-Thr-Tyr-Ser-Thr-Gly-Trp amide for N. vespilloides (code-named Nicve-AKH) and pGlu-Ile-Asn-Tyr-Ser-Thr-Gly-Trp amide for H. axyridis (code-named Harax-AKH). Moreover, synthetic peptides were shown to be active in the heterologous cockroach assay system, but Harax-AKH only at a dose of 30 pmol. It appears that the tyrosine residue at position 4 can be used as a diagnostic feature for certain bee

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA13-18509S" target="_blank" >GA13-18509S: Nové strategie značení alkylchlorformiáty pro cílenou a srovnávací metabolomiku technikami hmotnostní spektrometrie</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Amino Acids

ISSN

0939-4451

e-ISSN

—

Svazek periodika

47

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

11

Strana od-do

2323-2333

Kód UT WoS článku

000363481100007

EID výsledku v databázi Scopus

2-s2.0-84945450244