Carotenoid-protein interaction alters the S1 energy of hydroxyechinenone in the Orange Carotenoid Protein

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00395026" target="_blank" >RIV/60077344:_____/13:00395026 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/13:43885282

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.bbabio.2012.10.005" target="_blank" >http://dx.doi.org/10.1016/j.bbabio.2012.10.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbabio.2012.10.005" target="_blank" >10.1016/j.bbabio.2012.10.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Carotenoid-protein interaction alters the S1 energy of hydroxyechinenone in the Orange Carotenoid Protein

Popis výsledku v původním jazyce

We have determined the S1 energy of the carotenoid hydroxyechinenone (hECN) in organic solvent and compared it with the S1 energy of hECN bound to the OCP. In methanol and n-hexane, hECN has an S1 energy of 14,300 cm-1, slightly higher than carotenoids with shorter conjugation lengths such as zeaxanthin or beta-carotene; this is consistent with the proposal that the presence of the conjugated carbonyl group in hECN increases its S1 energy. Upon binding to the OCP, the S1 energy of hECN is further increased to 14,700 cm-1, underscoring the importance of protein binding which twists the conjugated carbonyl group into s-trans conformation and enhances the effect of the carbonyl group. Activated OCP, however, has an S1 energy of 14,000 cm-1, indicating that significant changes in the vicinity of the conjugated carbonyl group occur upon activation.

Název v anglickém jazyce

Carotenoid-protein interaction alters the S1 energy of hydroxyechinenone in the Orange Carotenoid Protein

Popis výsledku anglicky

We have determined the S1 energy of the carotenoid hydroxyechinenone (hECN) in organic solvent and compared it with the S1 energy of hECN bound to the OCP. In methanol and n-hexane, hECN has an S1 energy of 14,300 cm-1, slightly higher than carotenoids with shorter conjugation lengths such as zeaxanthin or beta-carotene; this is consistent with the proposal that the presence of the conjugated carbonyl group in hECN increases its S1 energy. Upon binding to the OCP, the S1 energy of hECN is further increased to 14,700 cm-1, underscoring the importance of protein binding which twists the conjugated carbonyl group into s-trans conformation and enhances the effect of the carbonyl group. Activated OCP, however, has an S1 energy of 14,000 cm-1, indicating that significant changes in the vicinity of the conjugated carbonyl group occur upon activation.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/GA202%2F09%2F1330" target="_blank" >GA202/09/1330: Excitované stavy karotenoidových agregátů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica Et Biophysica Acta-Bioenergetics

ISSN

0005-2728

e-ISSN

—

Svazek periodika

1827

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

248-254

Kód UT WoS článku

000315249000003

EID výsledku v databázi Scopus

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