Viability, longevity, and egg production of Drosophila melanogaster are regulated by the miR-282 microDNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00422599" target="_blank" >RIV/60077344:_____/13:00422599 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1534/genetics.113.153585" target="_blank" >http://dx.doi.org/10.1534/genetics.113.153585</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1534/genetics.113.153585" target="_blank" >10.1534/genetics.113.153585</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Viability, longevity, and egg production of Drosophila melanogaster are regulated by the miR-282 microDNA

Popis výsledku v původním jazyce

The first microRNAs were discovered some 20 years ago, but only a small fraction of the microRNA-encoding genes have been described in detail yet. Here we report the molecular analysis of a computationally predicted Drosophila melanogaster microRNA gene,mir-282. We show that the mir-282 gene is the source of a 4.9-kb-long primary transcript with a 5' cap and a 3'-poly(A) sequence and a mature microRNA of similar to 25 bp. Our data strongly suggest the existence of an independent mir-282 gene conservedin holometabolic insects. We give evidence that the mir-282 locus encodes a functional transcript that influences viability, longevity, and egg production in Drosophila. We identify the nervous system-specific adenylate cyclase (rutabaga) as a target ofmiR-282 and assume that one of the main functions of mir-282 is the regulation of adenylate cyclase activity in the nervous system during metamorphosis.

Název v anglickém jazyce

Viability, longevity, and egg production of Drosophila melanogaster are regulated by the miR-282 microDNA

Popis výsledku anglicky

The first microRNAs were discovered some 20 years ago, but only a small fraction of the microRNA-encoding genes have been described in detail yet. Here we report the molecular analysis of a computationally predicted Drosophila melanogaster microRNA gene,mir-282. We show that the mir-282 gene is the source of a 4.9-kb-long primary transcript with a 5' cap and a 3'-poly(A) sequence and a mature microRNA of similar to 25 bp. Our data strongly suggest the existence of an independent mir-282 gene conservedin holometabolic insects. We give evidence that the mir-282 locus encodes a functional transcript that influences viability, longevity, and egg production in Drosophila. We identify the nervous system-specific adenylate cyclase (rutabaga) as a target ofmiR-282 and assume that one of the main functions of mir-282 is the regulation of adenylate cyclase activity in the nervous system during metamorphosis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Genetics

ISSN

0016-6731

e-ISSN

—

Svazek periodika

195

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

469-480

Kód UT WoS článku

000325286200016

EID výsledku v databázi Scopus

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