Nucleotide Excision Repair Is Not Induced in Human Embryonic Lung Fibroblasts Treated with Environmental Pollutants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00425592" target="_blank" >RIV/60077344:_____/13:00425592 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378041:_____/13:00395392

Výsledek na webu

<a href="http://dx.doi.org/10.1371/journal.pone.0069197" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0069197</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0069197" target="_blank" >10.1371/journal.pone.0069197</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Nucleotide Excision Repair Is Not Induced in Human Embryonic Lung Fibroblasts Treated with Environmental Pollutants

Popis výsledku v původním jazyce

We tested response of normal human embryonic lung fibroblasts (HEL12469 cells) to treatment with benzo[a]pyrene (B[a]P) and extractable organic matter (EOM) from ambient air particles (PM2.5) collected in two Czech cities differing in levels and sourcesof air pollution. We analyzed multiple endpoints associated with exposure to PAHs: bulky DNA adducts and the nucleotide excision repair (NER) response [expression of XPE, XPC and XPA genes on the level of mRNA and proteins, unscheduled DNA synthesis (UDS)]. EOMs were collected in the winter and summer of 2011 in two Czech cities with different levels and sources of air pollution. The levels of bulky DNA adducts were highest after treatment with B[a]P, followed by winter EOMs; their induction by summer EOMs was weak. The induction of both mRNA and protein expression was observed, with the most pronounced effects after treatment with B[a]P (?S9); the response induced by EOMs from both cities and seasons was substantially weaker. The expre

Název v anglickém jazyce

Nucleotide Excision Repair Is Not Induced in Human Embryonic Lung Fibroblasts Treated with Environmental Pollutants

Popis výsledku anglicky

We tested response of normal human embryonic lung fibroblasts (HEL12469 cells) to treatment with benzo[a]pyrene (B[a]P) and extractable organic matter (EOM) from ambient air particles (PM2.5) collected in two Czech cities differing in levels and sourcesof air pollution. We analyzed multiple endpoints associated with exposure to PAHs: bulky DNA adducts and the nucleotide excision repair (NER) response [expression of XPE, XPC and XPA genes on the level of mRNA and proteins, unscheduled DNA synthesis (UDS)]. EOMs were collected in the winter and summer of 2011 in two Czech cities with different levels and sources of air pollution. The levels of bulky DNA adducts were highest after treatment with B[a]P, followed by winter EOMs; their induction by summer EOMs was weak. The induction of both mRNA and protein expression was observed, with the most pronounced effects after treatment with B[a]P (?S9); the response induced by EOMs from both cities and seasons was substantially weaker. The expre

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP503%2F11%2F0084" target="_blank" >GAP503/11/0084: Vliv polycyklických aromatických uhlovodíků a prachových částic na poškození DNA a na mechanismy reparace DNA in vitro</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS ONE

ISSN

1932-6203

e-ISSN

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Svazek periodika

8

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

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Kód UT WoS článku

000322391400043

EID výsledku v databázi Scopus

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