Cost effective, robust, and reliable coupled separation techniques for the identification and quantification of phospholipids in complex biological matrices: Application to insects

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F14%3A00429865" target="_blank" >RIV/60077344:_____/14:00429865 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388963:_____/14:00429865 RIV/60076658:12310/14:43887150

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201400113/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201400113/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201400113" target="_blank" >10.1002/jssc.201400113</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cost effective, robust, and reliable coupled separation techniques for the identification and quantification of phospholipids in complex biological matrices: Application to insects

Popis výsledku v původním jazyce

The quantification of phospholipid classes and the determination of their molecular structures are crucial in physiological and medical studies. This paper?s target analytes are cell membrane phospholipids, which play an important role in the seasonal acclimation processes of poikilothermic organisms.Weintroduce a set of simple and cost-effective analytical methods that enable efficient characterization and quantification of particular phospholipid classes and the identification and relative distribution of the individual phospholipid species. The analytical approach involves solid-phase extraction and high-performance thin-layer chromatography, which facilitate the separation of particular lipid classes. The obtained fractions are further transesterified to fatty acid methyl esters and subjected to gas chromatography coupled to flame ionization detection, which enables the determination of the position of double bonds. Phospholipid species separation is achieved by high-performance li

Název v anglickém jazyce

Cost effective, robust, and reliable coupled separation techniques for the identification and quantification of phospholipids in complex biological matrices: Application to insects

Popis výsledku anglicky

The quantification of phospholipid classes and the determination of their molecular structures are crucial in physiological and medical studies. This paper?s target analytes are cell membrane phospholipids, which play an important role in the seasonal acclimation processes of poikilothermic organisms.Weintroduce a set of simple and cost-effective analytical methods that enable efficient characterization and quantification of particular phospholipid classes and the identification and relative distribution of the individual phospholipid species. The analytical approach involves solid-phase extraction and high-performance thin-layer chromatography, which facilitate the separation of particular lipid classes. The obtained fractions are further transesterified to fatty acid methyl esters and subjected to gas chromatography coupled to flame ionization detection, which enables the determination of the position of double bonds. Phospholipid species separation is achieved by high-performance li

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

37

Číslo periodika v rámci svazku

15

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

2062-2068

Kód UT WoS článku

000339670600021

EID výsledku v databázi Scopus

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