Relaxation of the non-photochemical chlorophyll fluorescence quenching in diatoms: kinetics, components and mechanisms

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F14%3A00431328" target="_blank" >RIV/60077344:_____/14:00431328 - isvavai.cz</a>

Výsledek na webu

<a href="http://rstb.royalsocietypublishing.org" target="_blank" >http://rstb.royalsocietypublishing.org</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1098/rstb.2013.0241" target="_blank" >10.1098/rstb.2013.0241</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Relaxation of the non-photochemical chlorophyll fluorescence quenching in diatoms: kinetics, components and mechanisms

Popis výsledku v původním jazyce

In this contribution, the relaxation mechanisms of the non-photochemical Chl fluorescence quenching (qN) in diatoms are analyzed. An original nonlinear regression analysis of qN was used to obtain experimental evidences for the existence of three time-resolved qN components in the diatom Phaeodactylum tricornutum, namely qNf, qNi and qNs. qNf (s time-scale) and qNs (h time-scale) are exponential in shape, as qNi (min time-scale) is of sigmoidal nature and is dominant among the three components. Application of metabolic inhibitors (dithiothreitol, ammonium chloride, cadmium and diphenyleneiodonium chloride) allowed the identification of mechanisms on which each component mostly relies. qNi is linked to the relaxation of the pH gradient and the reversalof the xanthophyll cycle. qNs quantifies the stage of photoinhibition caused by the high light exposure, qNf seems to reflect fast conformational changes within thylakoid membranes in the vicinity of the photosystem II complexes.

Název v anglickém jazyce

Relaxation of the non-photochemical chlorophyll fluorescence quenching in diatoms: kinetics, components and mechanisms

Popis výsledku anglicky

In this contribution, the relaxation mechanisms of the non-photochemical Chl fluorescence quenching (qN) in diatoms are analyzed. An original nonlinear regression analysis of qN was used to obtain experimental evidences for the existence of three time-resolved qN components in the diatom Phaeodactylum tricornutum, namely qNf, qNi and qNs. qNf (s time-scale) and qNs (h time-scale) are exponential in shape, as qNi (min time-scale) is of sigmoidal nature and is dominant among the three components. Application of metabolic inhibitors (dithiothreitol, ammonium chloride, cadmium and diphenyleneiodonium chloride) allowed the identification of mechanisms on which each component mostly relies. qNi is linked to the relaxation of the pH gradient and the reversalof the xanthophyll cycle. qNs quantifies the stage of photoinhibition caused by the high light exposure, qNf seems to reflect fast conformational changes within thylakoid membranes in the vicinity of the photosystem II complexes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Philosophical Transactions of the Royal Society B - Biological Sciences

ISSN

0962-8436

e-ISSN

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Svazek periodika

369

Číslo periodika v rámci svazku

1640

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000332468400015

EID výsledku v databázi Scopus

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