Mapping of single-copy genes by TSA-FISH in th codling moth, Cydia pomonella

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F14%3A00436606" target="_blank" >RIV/60077344:_____/14:00436606 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/14:43887954

Výsledek na webu

<a href="http://www.biomedcentral.com/content/pdf/1471-2156-15-S2-S15.pdf" target="_blank" >http://www.biomedcentral.com/content/pdf/1471-2156-15-S2-S15.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1471-2156-15-S2-S15" target="_blank" >10.1186/1471-2156-15-S2-S15</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Mapping of single-copy genes by TSA-FISH in th codling moth, Cydia pomonella

Popis výsledku v původním jazyce

In this study, we adapted a FISH protocol with tyramide signal amplification (TSA-FISH) for detection of single-copy genes in Lepidoptera. We tested the protocol with probes prepared from partial sequences of Z-linked genes in the codling moth. Using a modified TSA-FISH protocol we successfully mapped a partial sequence of the Acetylcholinesterase 1 (Ace-1) gene to the Z chromosome and confirmed thus its Z-linkage. A subsequent combination of BAC-FISH with BAC probes containing anticipated neighbouringZ-linked genes and TSA-FISH with the Ace-1 probe allowed the integration of Ace-1 in the physical map of the codling moth Z chromosome. We also developed a two-colour TSA-FISH protocol which enabled us simultaneous localization of two Z-linked genes, Ace-1 and Notch, to the expected regions of the Z chromosome. Our results suggest that TSA-FISH represents a reliable technique for physical mapping of genes or localization of transgene cassettes on chromosomes of moths and butterflies.

Název v anglickém jazyce

Mapping of single-copy genes by TSA-FISH in th codling moth, Cydia pomonella

Popis výsledku anglicky

In this study, we adapted a FISH protocol with tyramide signal amplification (TSA-FISH) for detection of single-copy genes in Lepidoptera. We tested the protocol with probes prepared from partial sequences of Z-linked genes in the codling moth. Using a modified TSA-FISH protocol we successfully mapped a partial sequence of the Acetylcholinesterase 1 (Ace-1) gene to the Z chromosome and confirmed thus its Z-linkage. A subsequent combination of BAC-FISH with BAC probes containing anticipated neighbouringZ-linked genes and TSA-FISH with the Ace-1 probe allowed the integration of Ace-1 in the physical map of the codling moth Z chromosome. We also developed a two-colour TSA-FISH protocol which enabled us simultaneous localization of two Z-linked genes, Ace-1 and Notch, to the expected regions of the Z chromosome. Our results suggest that TSA-FISH represents a reliable technique for physical mapping of genes or localization of transgene cassettes on chromosomes of moths and butterflies.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

B M C Genetics

ISSN

1471-2156

e-ISSN

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Svazek periodika

15

Číslo periodika v rámci svazku

Supplement 2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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