Induction and suppression of tick cell antiviral RNAi responses by tick-borne flaviviruses

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F14%3A00439555" target="_blank" >RIV/60077344:_____/14:00439555 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/14:43887340

Výsledek na webu

<a href="http://dx.doi.org/10.1093/nar/gku657" target="_blank" >http://dx.doi.org/10.1093/nar/gku657</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gku657" target="_blank" >10.1093/nar/gku657</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Induction and suppression of tick cell antiviral RNAi responses by tick-borne flaviviruses

Popis výsledku v původním jazyce

Arboviruses are transmitted by distantly related arthropod vectors such as mosquitoes (class In-secta) and ticks (class Arachnida). RNA interference (RNAi) is the major antiviral mechanism in arthropods against arboviruses. Unlike in mosquitoes, tick antiviral RNAi is not understood, although this information is important to compare arbovirus/host interactions in different classes of arbovirus vectos. Using an Ixodes scapularis-derived cell line, key Argonaute proteins involved in RNAi and the responseagainst tick-borne Langat virus (Flaviviridae) replication were identified and phylogenetic relationships characterized. Analysis of small RNAs in infected cells showed the production of virus-derived small interfering RNAs (viRNAs), which are key molecules of the antiviral RNAi response. Importantly, viRNAs were longer (22 nucleotides) than those from other arbovirus vectors and mapped at highest frequency to the termini of the viral genome, as opposed to mosquito-borne flaviviruses. Mo

Název v anglickém jazyce

Induction and suppression of tick cell antiviral RNAi responses by tick-borne flaviviruses

Popis výsledku anglicky

Arboviruses are transmitted by distantly related arthropod vectors such as mosquitoes (class In-secta) and ticks (class Arachnida). RNA interference (RNAi) is the major antiviral mechanism in arthropods against arboviruses. Unlike in mosquitoes, tick antiviral RNAi is not understood, although this information is important to compare arbovirus/host interactions in different classes of arbovirus vectos. Using an Ixodes scapularis-derived cell line, key Argonaute proteins involved in RNAi and the responseagainst tick-borne Langat virus (Flaviviridae) replication were identified and phylogenetic relationships characterized. Analysis of small RNAs in infected cells showed the production of virus-derived small interfering RNAs (viRNAs), which are key molecules of the antiviral RNAi response. Importantly, viRNAs were longer (22 nucleotides) than those from other arbovirus vectors and mapped at highest frequency to the termini of the viral genome, as opposed to mosquito-borne flaviviruses. Mo

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP302%2F12%2F2490" target="_blank" >GAP302/12/2490: Interakce viru klíšťové encefalitidy s hostitelem na molekulární, buněčné a organismální úrovni</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Svazek periodika

42

Číslo periodika v rámci svazku

14

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

9436-9446

Kód UT WoS článku

000343219200056

EID výsledku v databázi Scopus

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