Transferable antibiotic resistance plasmids from biogas plant digestates often belong to the IncP-1 epsilon subgroup

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00444547" target="_blank" >RIV/60077344:_____/15:00444547 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.3389/fmicb.2014.00765" target="_blank" >http://dx.doi.org/10.3389/fmicb.2014.00765</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmicb.2014.00765" target="_blank" >10.3389/fmicb.2014.00765</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Transferable antibiotic resistance plasmids from biogas plant digestates often belong to the IncP-1 epsilon subgroup

Popis výsledku v původním jazyce

Manure is known to contain residues of antibiotics administered to farm animals as well as bacteria carrying antibiotic resistance genes (ARGs). These genes are often located on mobile genetic elements. In biogas plants (BGPs), organic substrates such asmanure and plant material are mixed and fermented in order to provide energy, and resulting digestates are used for soil fertilization. The fate of plasmid carrying bacteria from manure during the fermentation process is unknown. The present study focused on transferable antibiotic resistance plasmids from digestates of seven BGPs, using manure as a co-substrate, and their phenotypic and genotypic characterization. Plasmids conferring resistance to either tetracycline or sulfadiazine were captured by means of exogenous plasmid isolation from digestates into Pseudomonas putida KT2442 and Escherichia coli CV601 recipients, at transfer frequencies ranging from 10(-5) to 10(-7). Transconjugants (n = 101) were screened by PCR-Southern blot

Název v anglickém jazyce

Transferable antibiotic resistance plasmids from biogas plant digestates often belong to the IncP-1 epsilon subgroup

Popis výsledku anglicky

Manure is known to contain residues of antibiotics administered to farm animals as well as bacteria carrying antibiotic resistance genes (ARGs). These genes are often located on mobile genetic elements. In biogas plants (BGPs), organic substrates such asmanure and plant material are mixed and fermented in order to provide energy, and resulting digestates are used for soil fertilization. The fate of plasmid carrying bacteria from manure during the fermentation process is unknown. The present study focused on transferable antibiotic resistance plasmids from digestates of seven BGPs, using manure as a co-substrate, and their phenotypic and genotypic characterization. Plasmids conferring resistance to either tetracycline or sulfadiazine were captured by means of exogenous plasmid isolation from digestates into Pseudomonas putida KT2442 and Escherichia coli CV601 recipients, at transfer frequencies ranging from 10(-5) to 10(-7). Transconjugants (n = 101) were screened by PCR-Southern blot

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/EE2.3.30.0032" target="_blank" >EE2.3.30.0032: Vytvoření postdoktorandských pozic na Biologickém centru AV ČR k rozvoji biologických disciplín a dosažení globální konkurenceschopnosti</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Microbiology

ISSN

1664-302X

e-ISSN

—

Svazek periodika

5

Číslo periodika v rámci svazku

January

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

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Kód UT WoS článku

000348859400001

EID výsledku v databázi Scopus

2-s2.0-84927518366