Tetracycline resistance genes persist in soil amended with cattle feces independently from chlortetracycline selection pressure

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00444553" target="_blank" >RIV/60077344:_____/15:00444553 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67179843:_____/15:00444553

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.soilbio.2014.11.018" target="_blank" >http://dx.doi.org/10.1016/j.soilbio.2014.11.018</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.soilbio.2014.11.018" target="_blank" >10.1016/j.soilbio.2014.11.018</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Tetracycline resistance genes persist in soil amended with cattle feces independently from chlortetracycline selection pressure

Popis výsledku v původním jazyce

Antibiotic residues and antibiotic resistance genes originating from animal waste represent environmental pollutants with possible human health consequences. In this study, we addressed the question whether chlortetracycline (CTC) residues in soils can act as selective pressure enhancing the persistence of tetracycline (TC-r) resistance genes in grassland soils receiving cattle feces. We performed a soil microcosm experiment, using 3 grassland soils with different management history, which were incubated with feces from conventionally raised dairy cows. The microcosms included treatments with a low dose (0.2 mg kg-1), a high dose (100 mg kg-1) and no CTC. The presence and abundance of TC-r genes tet(O), tet(Q) and tet(W) and the intI1 gene coding for class 1 integrase were assessed with real-time PCR after 0, 14, 28, 56 and 86 d of incubation. The genes tet(Q) and intI1 persisted in all feces-containing treatments for at least 28 d, and tet(W) and tet(0) for at least 86 d, though they

Název v anglickém jazyce

Tetracycline resistance genes persist in soil amended with cattle feces independently from chlortetracycline selection pressure

Popis výsledku anglicky

Antibiotic residues and antibiotic resistance genes originating from animal waste represent environmental pollutants with possible human health consequences. In this study, we addressed the question whether chlortetracycline (CTC) residues in soils can act as selective pressure enhancing the persistence of tetracycline (TC-r) resistance genes in grassland soils receiving cattle feces. We performed a soil microcosm experiment, using 3 grassland soils with different management history, which were incubated with feces from conventionally raised dairy cows. The microcosms included treatments with a low dose (0.2 mg kg-1), a high dose (100 mg kg-1) and no CTC. The presence and abundance of TC-r genes tet(O), tet(Q) and tet(W) and the intI1 gene coding for class 1 integrase were assessed with real-time PCR after 0, 14, 28, 56 and 86 d of incubation. The genes tet(Q) and intI1 persisted in all feces-containing treatments for at least 28 d, and tet(W) and tet(0) for at least 86 d, though they

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Soil Biology and Biochemistry

ISSN

0038-0717

e-ISSN

—

Svazek periodika

81

Číslo periodika v rámci svazku

February

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

259-265

Kód UT WoS článku

000350524700031

EID výsledku v databázi Scopus

2-s2.0-84917671976