Molecular Characterization of Aster Yellows Subgroup 16SrI-B Phytoplasma in Verbena x hybrida

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00446120" target="_blank" >RIV/60077344:_____/15:00446120 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/jph.12303" target="_blank" >http://dx.doi.org/10.1111/jph.12303</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jph.12303" target="_blank" >10.1111/jph.12303</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular Characterization of Aster Yellows Subgroup 16SrI-B Phytoplasma in Verbena x hybrida

Popis výsledku v původním jazyce

Symptoms resembling phytoplasma disease were observed on Verbena x hybrida in Turkey in 2013. Infected plants had discoloured flowers, poor flower clusters, inflorescences with a small number of developed flowers and thickened fruit stalks. Electron microscopy examination showed phytoplasma-like bodies bodies in phloem cells of leaf midribs. Phytoplasma presence was confirmed by polymerase chain reaction of the 16S rRNA gene, the 16?23S rRNA intergenic spacer region and the start of the 23S rRNA gene using phytoplasma-specific primer pair P1A/P7A, two ribosomal protein (rp) genes (rpl22 and rps3) and the Tuf gene. Comparison of the amplified sequences with those available in GenBank allowed classification of the phytoplasma into aster yellows subgroups16SrI-B, rpI-B and tufI-B. This is the first report about molecular detection and identification of natural infection of the genus Verbena by phytoplasma.

Název v anglickém jazyce

Molecular Characterization of Aster Yellows Subgroup 16SrI-B Phytoplasma in Verbena x hybrida

Popis výsledku anglicky

Symptoms resembling phytoplasma disease were observed on Verbena x hybrida in Turkey in 2013. Infected plants had discoloured flowers, poor flower clusters, inflorescences with a small number of developed flowers and thickened fruit stalks. Electron microscopy examination showed phytoplasma-like bodies bodies in phloem cells of leaf midribs. Phytoplasma presence was confirmed by polymerase chain reaction of the 16S rRNA gene, the 16?23S rRNA intergenic spacer region and the start of the 23S rRNA gene using phytoplasma-specific primer pair P1A/P7A, two ribosomal protein (rp) genes (rpl22 and rps3) and the Tuf gene. Comparison of the amplified sequences with those available in GenBank allowed classification of the phytoplasma into aster yellows subgroups16SrI-B, rpI-B and tufI-B. This is the first report about molecular detection and identification of natural infection of the genus Verbena by phytoplasma.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Phytopathology - Phytopathologische Zeitschrift

ISSN

0931-1785

e-ISSN

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Svazek periodika

163

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

664-669

Kód UT WoS článku

000357465500019

EID výsledku v databázi Scopus

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