Electron beam induced mass loss dependence on aging of Epon resin sections

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00452276" target="_blank" >RIV/60077344:_____/15:00452276 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081731:_____/15:00452276

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electron beam induced mass loss dependence on aging of Epon resin sections

Popis výsledku v původním jazyce

It has been recently shown that low voltage STEM (Scanning Transmission Electron Microscope) can be regarded as the method of choice for many studies of biological samples. Main advantage of this technique is that it allows one to image samples with lowcontrast. Usually, in a standard STEM (typical acceleration voltage 60?300 kV) the image contrast is enhanced by staining using salts of heavy metals (e.g., uranyl acetate, lead citrate). This is not necessarily in low voltage STEM. Consequently, investigated structures are closer to native state of investigated samples. During sample preparation biological material is embedded in a resin which is observed in microscope in a form of ultrathin sections. There is commercially available wide spectrum of resins with different properties for embedding samples in a range from soft tissues to bones. They have various parameters such as hardness, mechanical stability, penetrability. For imaging an important parameter is a sensitivity to degrada

Název v anglickém jazyce

Electron beam induced mass loss dependence on aging of Epon resin sections

Popis výsledku anglicky

It has been recently shown that low voltage STEM (Scanning Transmission Electron Microscope) can be regarded as the method of choice for many studies of biological samples. Main advantage of this technique is that it allows one to image samples with lowcontrast. Usually, in a standard STEM (typical acceleration voltage 60?300 kV) the image contrast is enhanced by staining using salts of heavy metals (e.g., uranyl acetate, lead citrate). This is not necessarily in low voltage STEM. Consequently, investigated structures are closer to native state of investigated samples. During sample preparation biological material is embedded in a resin which is observed in microscope in a form of ultrathin sections. There is commercially available wide spectrum of resins with different properties for embedding samples in a range from soft tissues to bones. They have various parameters such as hardness, mechanical stability, penetrability. For imaging an important parameter is a sensitivity to degrada

Druh

D - Stať ve sborníku

CEP obor

JA - Elektronika a optoelektronika, elektrotechnika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

12th Multinational Congress on Microscopy

ISBN

978-963-05-9653-4

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

112-113

Název nakladatele

Akadémiai Kiadó

Místo vydání

Budapest

Místo konání akce

Eger

Datum konání akce

23. 8. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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