Genetic diversity of Cryptosporidium spp. including novel identification of the Cryptosporidium muris and Cryptosporidium tyzzeri in horses in the Czech Republic and Poland

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00453292" target="_blank" >RIV/60077344:_____/15:00453292 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/15:43889009 RIV/60076658:12510/15:43889009 RIV/60076658:12220/15:43889009

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00436-015-4353-y" target="_blank" >http://dx.doi.org/10.1007/s00436-015-4353-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00436-015-4353-y" target="_blank" >10.1007/s00436-015-4353-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Genetic diversity of Cryptosporidium spp. including novel identification of the Cryptosporidium muris and Cryptosporidium tyzzeri in horses in the Czech Republic and Poland

Popis výsledku v původním jazyce

Faecal samples were collected from 352 horses on 23 farms operating under six different management systems in the Czech Republic and Poland during 2011 and 2012. Farms were selected without previous knowledge of parasitological status. All faecal sampleswere screened for Cryptosporidium spp. presence using microscopy, following aniline-carbolmethyl violet staining and PCR analysis of the small-subunit (SSU) rRNA and the 60-kDa glycoprotein (gp60) genes. Cryptosporidium muris-positive samples were additionally genotyped at four minisatellite markers: MS1 (encoding a hypothetical protein), MS2 (encoding a 90-kDa heat shock protein), MS3 (encoding a hypothetical protein) and MS-16 (encoding a leucine-rich repeat family protein). Cryptosporidium spp. wasdetected by PCR in 12/352 (3.4 %) samples from 4 out of 13 farms. None of the samples tested by microscopy was positive. There was no relationship between Cryptosporidium prevalence and age, sex, diarrhoea or management system; however, C

Název v anglickém jazyce

Genetic diversity of Cryptosporidium spp. including novel identification of the Cryptosporidium muris and Cryptosporidium tyzzeri in horses in the Czech Republic and Poland

Popis výsledku anglicky

Faecal samples were collected from 352 horses on 23 farms operating under six different management systems in the Czech Republic and Poland during 2011 and 2012. Farms were selected without previous knowledge of parasitological status. All faecal sampleswere screened for Cryptosporidium spp. presence using microscopy, following aniline-carbolmethyl violet staining and PCR analysis of the small-subunit (SSU) rRNA and the 60-kDa glycoprotein (gp60) genes. Cryptosporidium muris-positive samples were additionally genotyped at four minisatellite markers: MS1 (encoding a hypothetical protein), MS2 (encoding a 90-kDa heat shock protein), MS3 (encoding a hypothetical protein) and MS-16 (encoding a leucine-rich repeat family protein). Cryptosporidium spp. wasdetected by PCR in 12/352 (3.4 %) samples from 4 out of 13 farms. None of the samples tested by microscopy was positive. There was no relationship between Cryptosporidium prevalence and age, sex, diarrhoea or management system; however, C

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/GA15-01090S" target="_blank" >GA15-01090S: Rozkrývání rozmanitosti kryptosporidií: propojení studia genetické variability a biologie parazitů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasitology Research

ISSN

0932-0113

e-ISSN

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Svazek periodika

114

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

1619-1624

Kód UT WoS článku

000351523900038

EID výsledku v databázi Scopus

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