Bioenergetics of the Bloodstream T. brucei Mitochondrion: a New Look

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00488328" target="_blank" >RIV/60077344:_____/15:00488328 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.parazitologie.cz/protozoologie/Protodny2015/JPD_sbornik_2015.pdf" target="_blank" >http://www.parazitologie.cz/protozoologie/Protodny2015/JPD_sbornik_2015.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Bioenergetics of the Bloodstream T. brucei Mitochondrion: a New Look

Popis výsledku v původním jazyce

The infective bloodstream stage (BS) of Trypanosoma brucei possesses a single mitochondrion that lacks a cytochrome-mediated respiratory chain and thus employs the hydrolytic activity of FoF1-ATPase to maintain the essential mitochondrial (mt) membrane potential (m). Meanwhile, dyskinetoplastic (Dk) trypanosomes lacking the mt encoded A6 that is essential for the functional Fo proton pore alternatively maintain their m by combining the hydrolytic activity of the matrix-facing F1-ATPase and the electrogenic exchange of ATP4- for ADP3- by the ADP/ATP carrier (AAC). Interestingly, the EC50 values of AAC inhibitor atractyloside are approximately 100× higher in BS cells compared to EC50 values measured for Dk trypanosomes. This result would suggest that AAC activity is not as important for BS as for Dk cells and thus the ATP for maintaining the m in BS cells is provided by mt substrate phosphorylation pathway(s). Indeed, RNAi silencing of AAC in BS trypanosomes has no effect on growth in vitro. The alternative sources of mt ATP will be discussed and revisited mt energy metabolism map of the infectious stage of the parasite will be presented.

Název v anglickém jazyce

Bioenergetics of the Bloodstream T. brucei Mitochondrion: a New Look

Popis výsledku anglicky

The infective bloodstream stage (BS) of Trypanosoma brucei possesses a single mitochondrion that lacks a cytochrome-mediated respiratory chain and thus employs the hydrolytic activity of FoF1-ATPase to maintain the essential mitochondrial (mt) membrane potential (m). Meanwhile, dyskinetoplastic (Dk) trypanosomes lacking the mt encoded A6 that is essential for the functional Fo proton pore alternatively maintain their m by combining the hydrolytic activity of the matrix-facing F1-ATPase and the electrogenic exchange of ATP4- for ADP3- by the ADP/ATP carrier (AAC). Interestingly, the EC50 values of AAC inhibitor atractyloside are approximately 100× higher in BS cells compared to EC50 values measured for Dk trypanosomes. This result would suggest that AAC activity is not as important for BS as for Dk cells and thus the ATP for maintaining the m in BS cells is provided by mt substrate phosphorylation pathway(s). Indeed, RNAi silencing of AAC in BS trypanosomes has no effect on growth in vitro. The alternative sources of mt ATP will be discussed and revisited mt energy metabolism map of the infectious stage of the parasite will be presented.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/LL1205" target="_blank" >LL1205: Charakterizace unikátních vlastností esenciální FoF1 ATP syntázy u původce africké spavé nemoci Trypanosoma bucei za účelem vývoje inhibitorů tohoto komplexu.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů