Profiling of urinary amino-carboxylic metabolites by in-situ heptafluorobutyl chloroformate mediated sample preparation and gas chromatography-mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F16%3A00458358" target="_blank" >RIV/60077344:_____/16:00458358 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00843989:_____/16:E0105395

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0021967316302692" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0021967316302692</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2016.03.019" target="_blank" >10.1016/j.chroma.2016.03.019</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Profiling of urinary amino-carboxylic metabolites by in-situ heptafluorobutyl chloroformate mediated sample preparation and gas chromatography-mass spectrometry

Popis výsledku v původním jazyce

A novel heptafluorobutyl chloroformate reagent (HFBCF) was examined for in-situ derivatization of amino-carboxylic metabolites in human urine. The sample preparation protocol provides cleaner extracts than other urinary GC–MS based methods. Reaction products of 153 urinary metabolites with HFBCF and two internal standards were investigated in detail by GC–MS and LC–MS. The new method was validated for 132 metabolites in artificial urine with special attention to potential disease biomarker candidates. The sample preparation protocol was finally evaluated by means of a certified organic acid standard mixture in urine and by GC–MS analysis of 100 morning urines obtained from healthy patients, where 112 physiological metabolites were quantified in a 25 µL sample aliquot. Most metabolites were found within the range reported in the reference human metabolome (HMDB) database and literature. The described method has been a promising tool for targeted GC–MS based metabolomic analysis in urine.n

Název v anglickém jazyce

Profiling of urinary amino-carboxylic metabolites by in-situ heptafluorobutyl chloroformate mediated sample preparation and gas chromatography-mass spectrometry

Popis výsledku anglicky

A novel heptafluorobutyl chloroformate reagent (HFBCF) was examined for in-situ derivatization of amino-carboxylic metabolites in human urine. The sample preparation protocol provides cleaner extracts than other urinary GC–MS based methods. Reaction products of 153 urinary metabolites with HFBCF and two internal standards were investigated in detail by GC–MS and LC–MS. The new method was validated for 132 metabolites in artificial urine with special attention to potential disease biomarker candidates. The sample preparation protocol was finally evaluated by means of a certified organic acid standard mixture in urine and by GC–MS analysis of 100 morning urines obtained from healthy patients, where 112 physiological metabolites were quantified in a 25 µL sample aliquot. Most metabolites were found within the range reported in the reference human metabolome (HMDB) database and literature. The described method has been a promising tool for targeted GC–MS based metabolomic analysis in urine.n

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA13-18509S" target="_blank" >GA13-18509S: Nové strategie značení alkylchlorformiáty pro cílenou a srovnávací metabolomiku technikami hmotnostní spektrometrie</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1443

Číslo periodika v rámci svazku

April 22

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

22

Strana od-do

211-232

Kód UT WoS článku

000374362600025

EID výsledku v databázi Scopus

2-s2.0-84977962792