Identification and bioactivity evaluation of the first neuropeptide from the lesser-known insect order Embioptera (webspinner)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F16%3A00459064" target="_blank" >RIV/60077344:_____/16:00459064 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/article/10.1007%2Fs00726-016-2229-9" target="_blank" >http://link.springer.com/article/10.1007%2Fs00726-016-2229-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00726-016-2229-9" target="_blank" >10.1007/s00726-016-2229-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification and bioactivity evaluation of the first neuropeptide from the lesser-known insect order Embioptera (webspinner)

Popis výsledku v původním jazyce

A species of the poorly studied order Embioptera, the webspinner Oligotoma saundersii, was investigated for the peptides of the adipokinetic hormone (AKH) family. A methanolic extract of its corpora cardiaca (CC) effects carbohydrate mobilization in the cockroach, Periplaneta americana, and LC-ESI MS analysis revealed one decapeptide elucidated as pEVNFSPNWGG amide in the CC of O. saundersii. It is a novel member of the AKH family and its synthetic form has strong hypertrehalosemic activity in the American cockroach. An analog with the reversed order of the proline and asparagine residues, viz. N6 P7 -Olisa-AKH, had negligible activity thus, sheddingnlight on the requirements of the cockroach AKH receptor. The CC from an individual webspinner contains less than one pmol of Olisa-AKH. Comparison of the AKH sequences from the major orders of the Polyneoptera does not point to a close phylogenetic relationship between webspinners and stick insects.

Název v anglickém jazyce

Identification and bioactivity evaluation of the first neuropeptide from the lesser-known insect order Embioptera (webspinner)

Popis výsledku anglicky

A species of the poorly studied order Embioptera, the webspinner Oligotoma saundersii, was investigated for the peptides of the adipokinetic hormone (AKH) family. A methanolic extract of its corpora cardiaca (CC) effects carbohydrate mobilization in the cockroach, Periplaneta americana, and LC-ESI MS analysis revealed one decapeptide elucidated as pEVNFSPNWGG amide in the CC of O. saundersii. It is a novel member of the AKH family and its synthetic form has strong hypertrehalosemic activity in the American cockroach. An analog with the reversed order of the proline and asparagine residues, viz. N6 P7 -Olisa-AKH, had negligible activity thus, sheddingnlight on the requirements of the cockroach AKH receptor. The CC from an individual webspinner contains less than one pmol of Olisa-AKH. Comparison of the AKH sequences from the major orders of the Polyneoptera does not point to a close phylogenetic relationship between webspinners and stick insects.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA13-18509S" target="_blank" >GA13-18509S: Nové strategie značení alkylchlorformiáty pro cílenou a srovnávací metabolomiku technikami hmotnostní spektrometrie</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Amino Acids

ISSN

0939-4451

e-ISSN

—

Svazek periodika

48

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

8

Strana od-do

1677-1684

Kód UT WoS článku

000377409900013

EID výsledku v databázi Scopus

2-s2.0-84963645414