Roles of the Nfu Fe–S targeting factors in the trypanosome mitochondrion

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F16%3A00463325" target="_blank" >RIV/60077344:_____/16:00463325 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/16:43891088

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ijpara.2016.04.006" target="_blank" >http://dx.doi.org/10.1016/j.ijpara.2016.04.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijpara.2016.04.006" target="_blank" >10.1016/j.ijpara.2016.04.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Roles of the Nfu Fe–S targeting factors in the trypanosome mitochondrion

Popis výsledku v původním jazyce

Iron-sulphur clusters (ISCs) are protein co-factors essential for a wide range of cellular functions. The core iron-sulphur cluster assembly machinery resides in the mitochondrion, yet due to export of an essential precursor from the organelle, it is also needed for cytosolic and nuclear iron-sulphur cluster assembly. In mitochondria all [4Fe-4S] iron-sulphur clusters are synthesised and transferred to specific apoproteins by so-called iron-sulphur cluster targeting factors. One of these factors is the universally present mitochondrial Nfu1, which in humans is required for the proper assembly of a subset of mitochondrial [4Fe-4S] proteins. Although most eukaryotes harbour a single Nfu1, the genomes of Trypanosoma brucei and related flagellates encode three Nfu genes. All three Nfu proteins localise to the mitochondrion in the procyclic form of T. brucei, and TbNfu2 and TbNfu3 are both individually essential for growth in bloodstream and procyclic forms, suggesting highly specific functions for each of these proteins in the trypanosome cell. Moreover, these two proteins are functional in the iron-sulphur cluster assembly in a heterologous system and rescue the growth defect of a yeast deletion mutant.

Název v anglickém jazyce

Roles of the Nfu Fe–S targeting factors in the trypanosome mitochondrion

Popis výsledku anglicky

Iron-sulphur clusters (ISCs) are protein co-factors essential for a wide range of cellular functions. The core iron-sulphur cluster assembly machinery resides in the mitochondrion, yet due to export of an essential precursor from the organelle, it is also needed for cytosolic and nuclear iron-sulphur cluster assembly. In mitochondria all [4Fe-4S] iron-sulphur clusters are synthesised and transferred to specific apoproteins by so-called iron-sulphur cluster targeting factors. One of these factors is the universally present mitochondrial Nfu1, which in humans is required for the proper assembly of a subset of mitochondrial [4Fe-4S] proteins. Although most eukaryotes harbour a single Nfu1, the genomes of Trypanosoma brucei and related flagellates encode three Nfu genes. All three Nfu proteins localise to the mitochondrion in the procyclic form of T. brucei, and TbNfu2 and TbNfu3 are both individually essential for growth in bloodstream and procyclic forms, suggesting highly specific functions for each of these proteins in the trypanosome cell. Moreover, these two proteins are functional in the iron-sulphur cluster assembly in a heterologous system and rescue the growth defect of a yeast deletion mutant.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

<a href="/cs/project/EE2.3.30.0006" target="_blank" >EE2.3.30.0006: Vytvoření postdoktorandských pozic na Jihočeské univerzitě a podpora intersektorální mobility formou odborných zahraničních stáží</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal for Parasitology

ISSN

0020-7519

e-ISSN

—

Svazek periodika

46

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

AU - Austrálie

Počet stran výsledku

11

Strana od-do

641-651

Kód UT WoS článku

000383303100004

EID výsledku v databázi Scopus

2-s2.0-84971607269