Post-transcriptional mending of gene sequences: looking under the hood of mitochondrial gene expression in diplonemids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F16%3A00468794" target="_blank" >RIV/60077344:_____/16:00468794 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1080/15476286.2016.1240143" target="_blank" >http://dx.doi.org/10.1080/15476286.2016.1240143</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/15476286.2016.1240143" target="_blank" >10.1080/15476286.2016.1240143</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Post-transcriptional mending of gene sequences: looking under the hood of mitochondrial gene expression in diplonemids

Popis výsledku v původním jazyce

The instructions to make proteins and structural RNAs are laid down in gene sequences. Yet, in certain instances, these primary instructions need to be modified considerably during gene expression, most often at the transcript level. Here we review a case of massive post-transcriptional revisions via trans-splicing and RNA editing, a phenomenon occurring in mitochondria of a recently recognized protist group, the diplonemids. As of now, the various post-transcriptional steps have been cataloged in detail, but how these processes function is still unknown. Since genetic manipulation techniques such as gene replacement and RNA interference have not yet been established for these organisms, alternative strategies have to be deployed. Here, we discuss the experimental and bioinformatics approaches that promise to unravel the molecular machineries of trans-splicing and RNA editing in Diplonema mitochondria.

Název v anglickém jazyce

Post-transcriptional mending of gene sequences: looking under the hood of mitochondrial gene expression in diplonemids

Popis výsledku anglicky

The instructions to make proteins and structural RNAs are laid down in gene sequences. Yet, in certain instances, these primary instructions need to be modified considerably during gene expression, most often at the transcript level. Here we review a case of massive post-transcriptional revisions via trans-splicing and RNA editing, a phenomenon occurring in mitochondria of a recently recognized protist group, the diplonemids. As of now, the various post-transcriptional steps have been cataloged in detail, but how these processes function is still unknown. Since genetic manipulation techniques such as gene replacement and RNA interference have not yet been established for these organisms, alternative strategies have to be deployed. Here, we discuss the experimental and bioinformatics approaches that promise to unravel the molecular machineries of trans-splicing and RNA editing in Diplonema mitochondria.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA15-21974S" target="_blank" >GA15-21974S: Studium vztahů mezi RNA-vážícími bílkovinami a mitochondriálním genomem trypanosom</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

RNA biology

ISSN

1547-6286

e-ISSN

—

Svazek periodika

13

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

1204-1211

Kód UT WoS článku

000390670300005

EID výsledku v databázi Scopus

2-s2.0-85003794187