HfMV1 and another putative mycovirus in Central European populations of Hymenoscyphus fraxineus, the causal agent of ash dieback in Europe

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00475031" target="_blank" >RIV/60077344:_____/17:00475031 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43410/17:43912748 RIV/62156489:43510/17:43912748

Výsledek na webu

<a href="https://www.balticforestry.mi.lt/bf/PDF_Articles/2017-23%5B1%5D/Baltic%20Forestry%202017.1_107-115.pdf" target="_blank" >https://www.balticforestry.mi.lt/bf/PDF_Articles/2017-23%5B1%5D/Baltic%20Forestry%202017.1_107-115.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

HfMV1 and another putative mycovirus in Central European populations of Hymenoscyphus fraxineus, the causal agent of ash dieback in Europe

Popis výsledku v původním jazyce

The occurrence of putative, doubled-stranded (ds) viral RNA particles was investigated in the decaying ascomycetous fungal pathogen Hymenoscyphus fraxineus. In total, 134 isolates obtained from 134 common European ashes (Fraxinus excelsior and Fraxinus angustifolia) were obtained from three European countries (Austria, Czech Republic and Slovakia). Three different dsRNA bands of ca 2.2, 2.5 and 4.5 kb were confirmed in 28.4 % of the examined H. fraxineus samples. The dsRNA band of ca 2.2 kb was the most frequent, with 21.6 % occurrence, while the ca 4.5 kb band was the least abundant, with 15.7 % occurrence. Complementary, Hymenoscyphus fraxineus mitovirus 1 (HfMV1) was recorded through high-throughput sequencing of dsRNA in one Czech isolate and confirmed to occur in all isolates presenting bands of ca 2.5 and/or 2.2 kb in size using direct specific retro-transcriptase (RT) PCR. The Czech mitovirus strain contained a single ORF of 2154 nt, encoding an RNA-dependent RNA polymerase (RdRp). These results confirm the presence of HfMV1 in Central Europe and provide evidence of a potential novel mycovirus in H. fraxineus.

Název v anglickém jazyce

HfMV1 and another putative mycovirus in Central European populations of Hymenoscyphus fraxineus, the causal agent of ash dieback in Europe

Popis výsledku anglicky

The occurrence of putative, doubled-stranded (ds) viral RNA particles was investigated in the decaying ascomycetous fungal pathogen Hymenoscyphus fraxineus. In total, 134 isolates obtained from 134 common European ashes (Fraxinus excelsior and Fraxinus angustifolia) were obtained from three European countries (Austria, Czech Republic and Slovakia). Three different dsRNA bands of ca 2.2, 2.5 and 4.5 kb were confirmed in 28.4 % of the examined H. fraxineus samples. The dsRNA band of ca 2.2 kb was the most frequent, with 21.6 % occurrence, while the ca 4.5 kb band was the least abundant, with 15.7 % occurrence. Complementary, Hymenoscyphus fraxineus mitovirus 1 (HfMV1) was recorded through high-throughput sequencing of dsRNA in one Czech isolate and confirmed to occur in all isolates presenting bands of ca 2.5 and/or 2.2 kb in size using direct specific retro-transcriptase (RT) PCR. The Czech mitovirus strain contained a single ORF of 2154 nt, encoding an RNA-dependent RNA polymerase (RdRp). These results confirm the presence of HfMV1 in Central Europe and provide evidence of a potential novel mycovirus in H. fraxineus.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10607 - Virology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Baltic Forestry

ISSN

1392-1355

e-ISSN

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Svazek periodika

23

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

LT - Litevská republika

Počet stran výsledku

9

Strana od-do

107-115

Kód UT WoS článku

000409024600013

EID výsledku v databázi Scopus

2-s2.0-85027335014