Hymenoscyphus fraxineus mitovirus 1 naturally disperses through the airborne inoculum of its host, Hymenoscyphus fraxineus, in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F17%3A43912653" target="_blank" >RIV/62156489:43410/17:43912653 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/17:00097502

Výsledek na webu

<a href="https://doi.org/10.1080/09583157.2017.1368455" target="_blank" >https://doi.org/10.1080/09583157.2017.1368455</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Hymenoscyphus fraxineus mitovirus 1 naturally disperses through the airborne inoculum of its host, Hymenoscyphus fraxineus, in the Czech Republic

Popis výsledku v původním jazyce

We investigated the ability of Hymenoscyphus fraxineus mitovirus 1 (HfMV1) to spread in nature through the airborne inoculum of its fungal host, the devastating Hymenoscyphus fraxineus. Eight rotating-arm spore traps were installed to collect the airborne spores (presumably ascospores) of H. fraxineus in four ecologically different ash forests in the Czech Republic. Spore trap samples were subjected to direct DNA and RNA extractions and subsequent qPCR and mitovirus-specific reverse transcription (RT) qPCR for detection and quantification of H. fraxineus and HfMV1, respectively. The prevalence of HfMV1 was confirmed in 57% of H. fraxineus sampled inocula, showing that its occurrence is significantly correlated with its fungal host H. fraxineus. Furthermore, the ratio of mitovirus carrying spores was shown to be constant at any level of H. fraxineus infection pressure independently of the studied locality. Temperatures of 19-21oC at the time of spore trapping correlated with the highest levels of both HfMV1 and H. fraxineus. Our data confirm H. fraxineus airborne inoculum as an efficient transport means for HfMV1 in nature. Moreover, this research uses a new technology to directly study a mycovirus through its host aerospora.

Název v anglickém jazyce

Hymenoscyphus fraxineus mitovirus 1 naturally disperses through the airborne inoculum of its host, Hymenoscyphus fraxineus, in the Czech Republic

Popis výsledku anglicky

We investigated the ability of Hymenoscyphus fraxineus mitovirus 1 (HfMV1) to spread in nature through the airborne inoculum of its fungal host, the devastating Hymenoscyphus fraxineus. Eight rotating-arm spore traps were installed to collect the airborne spores (presumably ascospores) of H. fraxineus in four ecologically different ash forests in the Czech Republic. Spore trap samples were subjected to direct DNA and RNA extractions and subsequent qPCR and mitovirus-specific reverse transcription (RT) qPCR for detection and quantification of H. fraxineus and HfMV1, respectively. The prevalence of HfMV1 was confirmed in 57% of H. fraxineus sampled inocula, showing that its occurrence is significantly correlated with its fungal host H. fraxineus. Furthermore, the ratio of mitovirus carrying spores was shown to be constant at any level of H. fraxineus infection pressure independently of the studied locality. Temperatures of 19-21oC at the time of spore trapping correlated with the highest levels of both HfMV1 and H. fraxineus. Our data confirm H. fraxineus airborne inoculum as an efficient transport means for HfMV1 in nature. Moreover, this research uses a new technology to directly study a mycovirus through its host aerospora.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10607 - Virology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biocontrol Science and Technology

ISSN

0958-3157

e-ISSN

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Svazek periodika

27

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

17

Strana od-do

992-1008

Kód UT WoS článku

000410578100007

EID výsledku v databázi Scopus

2-s2.0-85029081410