Vertical spread of Hymenoscyphus fraxineus propagules

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F23%3A43907832" target="_blank" >RIV/60076658:12220/23:43907832 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43410/23:43923481

Výsledek na webu

<a href="https://repozitar.mendelu.cz/xmlui/handle/20.500.12698/1759" target="_blank" >https://repozitar.mendelu.cz/xmlui/handle/20.500.12698/1759</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3897/neobiota.84.90981" target="_blank" >10.3897/neobiota.84.90981</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Vertical spread of Hymenoscyphus fraxineus propagules

Popis výsledku v původním jazyce

Currently, the ash dieback causal agent Hymenoscyphus fraxineus is an established invasive pathogen inmost European countries. Its potential to spread quickly among invaded forests is based on its propagules:airborne inoculum composed mainly of ascospores originated in apothecia growing on leaf litter infectedduring the previous vegetation season. The spread of the inoculum by air masses to distant areas is prob-able and depends on the availability of the ascospores in higher levels of air. Our study aimed to detect theinoculum in an infected area at heights of more than 20 meters. Our study was conducted in a municipallocality (Boršov nad Vltavou) with tens of infected ash trees (Fraxinus excelsior) in South Bohemia (SWCzechia). The infected trees surround an agricultural silo where five rotating arm spore traps (rotorods)were mounted for ten consequent 48h samplings during the peak of the sporulating season (17th July to 6thAugust 2020). The spore traps were mounted 48, 37, 25, 14 and 0,3 meters above ground. Samples werequantified by qPCR. Results clearly proved the ability of the spores to reach a height of 48 meters. Further-more, H. fraxineus DNA was detected from all five spore traps during all ten samplings. Mostly, the amountof detected spores showed a decreasing trend with height, and varied a lot. During some of the samplings,higher spore concetrations were achieved at the top than at the lower traps, which can be explained byhorizontal air transfer of the inoculum from other infected areas. Based on GLM analyses, higher sporeconcentrations were achieved during days without rain, lower air temperatures, after cloudy, humid andrainy weather without strong winds. A combination of rotorod ROTTRAP 52 with qPCR quantificationproved to be an efficient technology for a study focused on the vertical spread of H. fraxineus propagules.

Název v anglickém jazyce

Vertical spread of Hymenoscyphus fraxineus propagules

Popis výsledku anglicky

Currently, the ash dieback causal agent Hymenoscyphus fraxineus is an established invasive pathogen inmost European countries. Its potential to spread quickly among invaded forests is based on its propagules:airborne inoculum composed mainly of ascospores originated in apothecia growing on leaf litter infectedduring the previous vegetation season. The spread of the inoculum by air masses to distant areas is prob-able and depends on the availability of the ascospores in higher levels of air. Our study aimed to detect theinoculum in an infected area at heights of more than 20 meters. Our study was conducted in a municipallocality (Boršov nad Vltavou) with tens of infected ash trees (Fraxinus excelsior) in South Bohemia (SWCzechia). The infected trees surround an agricultural silo where five rotating arm spore traps (rotorods)were mounted for ten consequent 48h samplings during the peak of the sporulating season (17th July to 6thAugust 2020). The spore traps were mounted 48, 37, 25, 14 and 0,3 meters above ground. Samples werequantified by qPCR. Results clearly proved the ability of the spores to reach a height of 48 meters. Further-more, H. fraxineus DNA was detected from all five spore traps during all ten samplings. Mostly, the amountof detected spores showed a decreasing trend with height, and varied a lot. During some of the samplings,higher spore concetrations were achieved at the top than at the lower traps, which can be explained byhorizontal air transfer of the inoculum from other infected areas. Based on GLM analyses, higher sporeconcentrations were achieved during days without rain, lower air temperatures, after cloudy, humid andrainy weather without strong winds. A combination of rotorod ROTTRAP 52 with qPCR quantificationproved to be an efficient technology for a study focused on the vertical spread of H. fraxineus propagules.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10619 - Biodiversity conservation

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

NeoBiota

ISSN

1619-0033

e-ISSN

1314-2488

Svazek periodika

84

Číslo periodika v rámci svazku

2023

Stát vydavatele periodika

BG - Bulharská republika

Počet stran výsledku

16

Strana od-do

231-246

Kód UT WoS článku

001002757800010

EID výsledku v databázi Scopus

—