Larvae of Drosophila melanogaster exhibit transcriptional activation of immune response pathways and antimicrobial peptides during recovery from supercooling stress

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00500401" target="_blank" >RIV/60077344:_____/19:00500401 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/19:43899126

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0965174818304491?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0965174818304491?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ibmb.2019.01.006" target="_blank" >10.1016/j.ibmb.2019.01.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Larvae of Drosophila melanogaster exhibit transcriptional activation of immune response pathways and antimicrobial peptides during recovery from supercooling stress

Popis výsledku v původním jazyce

We focus on recovery from cold stress in the larvae of the vinegar fly (/Drosophila melanogaster/) that were exposed to two physiologically distinct cold stress situations: supercooling and freezing, both at -5°C. We analysed the metabolic and transcriptomic responses to cold stress via GC-MS / LC-MS and whole-genome microarrays, respectively. Both stresses caused metabolic perturbations which were transient in supercooled larvae but deeper and irreversible in frozen larvae. Using GO term enrichment analysis and KEGG pathway mapping, we identified the stimulation of immune response pathways as a strong candidate mechanism for coping with supercooling.The transcriptomic response to freezing was instead dominated by degradation of macromolecules and death-related processes such as autophagy and apoptosis.

Název v anglickém jazyce

Larvae of Drosophila melanogaster exhibit transcriptional activation of immune response pathways and antimicrobial peptides during recovery from supercooling stress

Popis výsledku anglicky

We focus on recovery from cold stress in the larvae of the vinegar fly (/Drosophila melanogaster/) that were exposed to two physiologically distinct cold stress situations: supercooling and freezing, both at -5°C. We analysed the metabolic and transcriptomic responses to cold stress via GC-MS / LC-MS and whole-genome microarrays, respectively. Both stresses caused metabolic perturbations which were transient in supercooled larvae but deeper and irreversible in frozen larvae. Using GO term enrichment analysis and KEGG pathway mapping, we identified the stimulation of immune response pathways as a strong candidate mechanism for coping with supercooling.The transcriptomic response to freezing was instead dominated by degradation of macromolecules and death-related processes such as autophagy and apoptosis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA16-06374S" target="_blank" >GA16-06374S: Poškození hmyzího organismu nízkou teplotou a jeho náprava.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Insect Biochemistry and Molecular Biology

ISSN

0965-1748

e-ISSN

—

Svazek periodika

105

Číslo periodika v rámci svazku

FEB 01

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

60-68

Kód UT WoS článku

000458943800007

EID výsledku v databázi Scopus

2-s2.0-85060335209