Casting light on Asgardarchaeota metabolism in a sunlit microoxic niche

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00509916" target="_blank" >RIV/60077344:_____/19:00509916 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.nature.com/articles/s41564-019-0404-y" target="_blank" >https://www.nature.com/articles/s41564-019-0404-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41564-019-0404-y" target="_blank" >10.1038/s41564-019-0404-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Casting light on Asgardarchaeota metabolism in a sunlit microoxic niche

Popis výsledku v původním jazyce

Recent advances in phylogenomic analyses and increased genomic sampling of uncultured prokaryotic lineages have brought compelling evidence in support of the emergence of eukaryotes from within the archaeal domain of life (eocyte hypothesis)(1,2). The discovery of Asgardarchaeota and its supposed position at the base of the eukaryotic tree of life(3,4) provided cues about the long-awaited identity of the eocytic lineage from which the nucleated cells (Eukaryota) emerged. While it is apparent that Asgardarchaeota encode a plethora of eukaryotic-specific proteins (the highest number identified yet in prokaryotes)(5), the lack of genomic information and metabolic characterization has precluded inferences about their lifestyles and the metabolic landscape that favoured the emergence of the protoeukaryote ancestor. Here, we use advanced phylogenetic analyses for inferring the deep ancestry of eukaryotes, and genome-scale metabolic reconstructions for shedding light on the metabolic milieu of Asgardarchaeota. In doing so, we: (1) show that Heimdallarchaeia (the closest eocytic lineage to eukaryotes to date) are likely to have a microoxic niche, based on their genomic potential, with aerobic metabolic pathways that are unique among Archaea (that is, the kynurenine pathway), (2) provide evidence of mixotrophy within Asgardarchaeota, and (3) describe a previously unknown family of rhodopsins encoded within the recovered genomes.

Název v anglickém jazyce

Casting light on Asgardarchaeota metabolism in a sunlit microoxic niche

Popis výsledku anglicky

Recent advances in phylogenomic analyses and increased genomic sampling of uncultured prokaryotic lineages have brought compelling evidence in support of the emergence of eukaryotes from within the archaeal domain of life (eocyte hypothesis)(1,2). The discovery of Asgardarchaeota and its supposed position at the base of the eukaryotic tree of life(3,4) provided cues about the long-awaited identity of the eocytic lineage from which the nucleated cells (Eukaryota) emerged. While it is apparent that Asgardarchaeota encode a plethora of eukaryotic-specific proteins (the highest number identified yet in prokaryotes)(5), the lack of genomic information and metabolic characterization has precluded inferences about their lifestyles and the metabolic landscape that favoured the emergence of the protoeukaryote ancestor. Here, we use advanced phylogenetic analyses for inferring the deep ancestry of eukaryotes, and genome-scale metabolic reconstructions for shedding light on the metabolic milieu of Asgardarchaeota. In doing so, we: (1) show that Heimdallarchaeia (the closest eocytic lineage to eukaryotes to date) are likely to have a microoxic niche, based on their genomic potential, with aerobic metabolic pathways that are unique among Archaea (that is, the kynurenine pathway), (2) provide evidence of mixotrophy within Asgardarchaeota, and (3) describe a previously unknown family of rhodopsins encoded within the recovered genomes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA17-04828S" target="_blank" >GA17-04828S: Objasnění životních strategií sladkovodních virů pomoci metagenomiky</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature Microbiology

ISSN

2058-5276

e-ISSN

—

Svazek periodika

4

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

1129-1137

Kód UT WoS článku

000480348200009

EID výsledku v databázi Scopus

2-s2.0-85063766324