NMR metabolomics reveals effects of Cryptosporidium infections on host cell metabolome

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00520226" target="_blank" >RIV/60077344:_____/19:00520226 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12220/19:43900296

Výsledek na webu

<a href="https://gutpathogens.biomedcentral.com/track/pdf/10.1186/s13099-019-0293-x" target="_blank" >https://gutpathogens.biomedcentral.com/track/pdf/10.1186/s13099-019-0293-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13099-019-0293-x" target="_blank" >10.1186/s13099-019-0293-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

NMR metabolomics reveals effects of Cryptosporidium infections on host cell metabolome

Popis výsledku v původním jazyce

BackgroundCryptosporidium is an important gut microbe whose contributions towards infant and immunocompromise patient mortality rates are steadily increasing. Over the last decade, we have seen the development of various tools and methods for studying Cryptosporidium infection and its interactions with their hosts. One area that is sorely overlooked is the effect infection has on host metabolic processes.ResultsUsing a H-1 nuclear magnetic resonance approach to metabolomics, we have explored the nature of the mouse gut metabolome as well as providing the first insight into the metabolome of an infected cell line. Statistical analysis and predictive modelling demonstrated new understandings of the effects of a Cryptosporidium infection, while verifying the presence of known metabolic changes. Of note is the potential contribution of host derived taurine to the diarrhoeal aspects of the disease previously attributed to a solely parasite-based alteration of the gut environment, in addition to other metabolites involved with host cell catabolism.ConclusionThis approach will spearhead our understanding of the Cryptosporidium-host metabolic exchange and provide novel targets for tackling this deadly parasite.

Název v anglickém jazyce

NMR metabolomics reveals effects of Cryptosporidium infections on host cell metabolome

Popis výsledku anglicky

BackgroundCryptosporidium is an important gut microbe whose contributions towards infant and immunocompromise patient mortality rates are steadily increasing. Over the last decade, we have seen the development of various tools and methods for studying Cryptosporidium infection and its interactions with their hosts. One area that is sorely overlooked is the effect infection has on host metabolic processes.ResultsUsing a H-1 nuclear magnetic resonance approach to metabolomics, we have explored the nature of the mouse gut metabolome as well as providing the first insight into the metabolome of an infected cell line. Statistical analysis and predictive modelling demonstrated new understandings of the effects of a Cryptosporidium infection, while verifying the presence of known metabolic changes. Of note is the potential contribution of host derived taurine to the diarrhoeal aspects of the disease previously attributed to a solely parasite-based alteration of the gut environment, in addition to other metabolites involved with host cell catabolism.ConclusionThis approach will spearhead our understanding of the Cryptosporidium-host metabolic exchange and provide novel targets for tackling this deadly parasite.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/GA15-01090S" target="_blank" >GA15-01090S: Rozkrývání rozmanitosti kryptosporidií: propojení studia genetické variability a biologie parazitů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Gut Pathogens

ISSN

1757-4749

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

APR 3 2019

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

13

Kód UT WoS článku

000463842900001

EID výsledku v databázi Scopus

2-s2.0-85063929769