Effects of Leishmania major infection on the gut microbiome of resistant and susceptible mice.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F24%3A00581728" target="_blank" >RIV/67985904:_____/24:00581728 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/24:00581728 RIV/68407700:21460/24:00373679 RIV/00216208:11120/24:43926564

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s00253-024-13002-y" target="_blank" >https://link.springer.com/article/10.1007/s00253-024-13002-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00253-024-13002-y" target="_blank" >10.1007/s00253-024-13002-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of Leishmania major infection on the gut microbiome of resistant and susceptible mice.

Popis výsledku v původním jazyce

Cutaneous leishmaniasis, a parasitic disease caused by Leishmania major, is a widely frequent form in humans. To explore the importance of the host gut microbiota and to investigate its changes during L. major infection, two different groups of mouse models were assessed. The microbiome of two parts of the host gut-ileum and colon-from infected and non-infected mice were characterised by sequencing of 16S rDNA using an Ion Torrent PGM platform. Microbiome analysis was performed to reveal changes related to the susceptibility and the genetics of mice strains in two different gut compartments and to compare the results between infected and non-infected mice. The results showed that Leishmania infection affects mainly the ileum microbiota, whereas the colon bacterial community was more stable. Different biomarkers were determined in the gut microbiota of infected resistant mice and infected susceptible mice using LEfSe analysis. Lactobacillaceae was associated with resistance in the colon microbiota of all resistant mice strains infected with L. major. Genes related to xenobiotic biodegradation and metabolism and amino acid metabolism were primarily enriched in the small intestine microbiome of resistant strains, while genes associated with carbohydrate metabolism and glycan biosynthesis and metabolism were most abundant in the gut microbiome of the infected susceptible mice. These results should improve our understanding of host-parasite interaction and provide important insights into the effect of leishmaniasis on the gut microbiota. Also, this study highlights the role of host genetic variation in shaping the diversity and composition of the gut microbiome. KEY POINTS: Leishmaniasis may affect mainly the ileum microbiota while colon microbiota was more stable. Biomarkers related with resistance or susceptibility were determined in the gut microbiota of mice. Several pathways were predicted to be upregulated in the gut microbiota of resistant or susceptible mice.

Název v anglickém jazyce

Effects of Leishmania major infection on the gut microbiome of resistant and susceptible mice.

Popis výsledku anglicky

Cutaneous leishmaniasis, a parasitic disease caused by Leishmania major, is a widely frequent form in humans. To explore the importance of the host gut microbiota and to investigate its changes during L. major infection, two different groups of mouse models were assessed. The microbiome of two parts of the host gut-ileum and colon-from infected and non-infected mice were characterised by sequencing of 16S rDNA using an Ion Torrent PGM platform. Microbiome analysis was performed to reveal changes related to the susceptibility and the genetics of mice strains in two different gut compartments and to compare the results between infected and non-infected mice. The results showed that Leishmania infection affects mainly the ileum microbiota, whereas the colon bacterial community was more stable. Different biomarkers were determined in the gut microbiota of infected resistant mice and infected susceptible mice using LEfSe analysis. Lactobacillaceae was associated with resistance in the colon microbiota of all resistant mice strains infected with L. major. Genes related to xenobiotic biodegradation and metabolism and amino acid metabolism were primarily enriched in the small intestine microbiome of resistant strains, while genes associated with carbohydrate metabolism and glycan biosynthesis and metabolism were most abundant in the gut microbiome of the infected susceptible mice. These results should improve our understanding of host-parasite interaction and provide important insights into the effect of leishmaniasis on the gut microbiota. Also, this study highlights the role of host genetic variation in shaping the diversity and composition of the gut microbiome. KEY POINTS: Leishmaniasis may affect mainly the ileum microbiota while colon microbiota was more stable. Biomarkers related with resistance or susceptibility were determined in the gut microbiota of mice. Several pathways were predicted to be upregulated in the gut microbiota of resistant or susceptible mice.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Microbiology and Biotechnology

ISSN

0175-7598

e-ISSN

1432-0614

Svazek periodika

108

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

16

Strana od-do

145

Kód UT WoS článku

001145802200003

EID výsledku v databázi Scopus

2-s2.0-85182850037