Estimation of influence of Leishmania major infection on mouse gut microbiomeby tools of molecular genetics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F18%3A00325938" target="_blank" >RIV/68407700:21460/18:00325938 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Estimation of influence of Leishmania major infection on mouse gut microbiomeby tools of molecular genetics

Popis výsledku v původním jazyce

Leishmaniasis is a disease caused by the protozoan parasite genus Leishmania. The disease is a current health problem in 98 countries worldwide. There is increasing knowledge about Leishmaniasis manifestation and its immunological background, but limited information about the microbiota dysbiosis caused by the disease. Gastrointestinal tract microbiota plays role in the development of immune system. The study was aimed to better understand the complex pathogenesis of Leishmania infection by examination of influence of Leishmania major on gut microbiome of mammalian host. The microbiome diversities were estimated by PCR-DGGE method, selected bands were identified by Sanger sequencing which led to dendrogram creation and cluster analysis. In the study a weight loss and lesions appeared in susceptible mice. There were bands identfied as Lactobacillus taiwanensis in colon samples. No significant linkage between strains was observed.

Název v anglickém jazyce

Estimation of influence of Leishmania major infection on mouse gut microbiomeby tools of molecular genetics

Popis výsledku anglicky

Leishmaniasis is a disease caused by the protozoan parasite genus Leishmania. The disease is a current health problem in 98 countries worldwide. There is increasing knowledge about Leishmaniasis manifestation and its immunological background, but limited information about the microbiota dysbiosis caused by the disease. Gastrointestinal tract microbiota plays role in the development of immune system. The study was aimed to better understand the complex pathogenesis of Leishmania infection by examination of influence of Leishmania major on gut microbiome of mammalian host. The microbiome diversities were estimated by PCR-DGGE method, selected bands were identified by Sanger sequencing which led to dendrogram creation and cluster analysis. In the study a weight loss and lesions appeared in susceptible mice. There were bands identfied as Lactobacillus taiwanensis in colon samples. No significant linkage between strains was observed.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů