Molecular genetics methods in use
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F20%3A00346181" target="_blank" >RIV/68407700:21460/20:00346181 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Molecular genetics methods in use
Popis výsledku v původním jazyce
The importance of studying microbial populations of the digestive tract in connection with various diseases is gaining relevance. Knowledge of the effect of leishmaniasis on the microbiome is limited. Genetically different individuals infected with the same species of parasite show a different composition of microbial populations of the digestive tract, which points to the importance of studying intestinal microbiota in relation to the host genotype. Aim of this study is a comparison of the composition of microbial populations of the digestive tract in health and disease depending on the host genotype. In the experimental model, we were able to define the effect of genetic background using several inbred strains in the chronic phase of infection and thus determine the effect of the disease itself. This work presents the molecular biology methods used for detailed analysis of immunopathological manifestations of Leishmania infection (PCR-ELISA) in combination with study of gut microbiome diversity (DGGE, NGS) in one experiment. These molecular genetics methods gave us a tool to prepare a more complex model of disease effects.
Název v anglickém jazyce
Molecular genetics methods in use
Popis výsledku anglicky
The importance of studying microbial populations of the digestive tract in connection with various diseases is gaining relevance. Knowledge of the effect of leishmaniasis on the microbiome is limited. Genetically different individuals infected with the same species of parasite show a different composition of microbial populations of the digestive tract, which points to the importance of studying intestinal microbiota in relation to the host genotype. Aim of this study is a comparison of the composition of microbial populations of the digestive tract in health and disease depending on the host genotype. In the experimental model, we were able to define the effect of genetic background using several inbred strains in the chronic phase of infection and thus determine the effect of the disease itself. This work presents the molecular biology methods used for detailed analysis of immunopathological manifestations of Leishmania infection (PCR-ELISA) in combination with study of gut microbiome diversity (DGGE, NGS) in one experiment. These molecular genetics methods gave us a tool to prepare a more complex model of disease effects.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů