Unique members of the adipokinetic hormone family in butterflies and moths (Insecta, Lepidoptera)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F20%3A00536352" target="_blank" >RIV/60077344:_____/20:00536352 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.frontiersin.org/articles/10.3389/fphys.2020.614552/pdf" target="_blank" >https://www.frontiersin.org/articles/10.3389/fphys.2020.614552/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fphys.2020.614552" target="_blank" >10.3389/fphys.2020.614552</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Unique members of the adipokinetic hormone family in butterflies and moths (Insecta, Lepidoptera)

Popis výsledku v původním jazyce

Lepidoptera is amongst one of the four most speciose insect orders and ecologically very successful because of their ability to fly. Insect flight is always aerobic and exactsna high metabolic demand on the animal. A family of structurally related neuropeptides, generically referred to as adipokinetic hormones (AKHs), play a key role in triggeringnthe release of readily utilizable fuel metabolites into the hemolymph from the storage forms in the fat body. We used mass spectrometry to elucidate AKH sequencesnfrom 34 species of Lepidoptera and searched the literature and publicly available databases to compile (in a phylogenetic context) a comprehensive list of all Lepidoptera sequences published/predicted from a total of 76 species. We then used the resulting set of 15 biochemically characterized AKHs in a physiological assay that measuresnlipid or carbohydrate mobilization in three different lepidopteran species to learn about the functional cross-activity (receptor-ligand interactions) amongst the differentnbutterfly/moth families. Our results include novel peptide structures, demonstrate structural diversity, phylogenetic trends in peptide distribution and order-specificitynof Lepidoptera AKHs. There is almost an equal occurrence of octa-, nona-, and decapeptides, with an unparalleled emphasis on nonapeptides than in any insect order.nPrimitive species make Peram-CAH-II, an octapeptide found also in other orders, the lepidopteran signature peptide is Manse-AKH. Not all of the 15 tested AKHs are activenin Pieris brassicae, this provides insight into structure-activity specificity and could be useful for further investigations into possible biorational insecticide development.

Název v anglickém jazyce

Unique members of the adipokinetic hormone family in butterflies and moths (Insecta, Lepidoptera)

Popis výsledku anglicky

Lepidoptera is amongst one of the four most speciose insect orders and ecologically very successful because of their ability to fly. Insect flight is always aerobic and exactsna high metabolic demand on the animal. A family of structurally related neuropeptides, generically referred to as adipokinetic hormones (AKHs), play a key role in triggeringnthe release of readily utilizable fuel metabolites into the hemolymph from the storage forms in the fat body. We used mass spectrometry to elucidate AKH sequencesnfrom 34 species of Lepidoptera and searched the literature and publicly available databases to compile (in a phylogenetic context) a comprehensive list of all Lepidoptera sequences published/predicted from a total of 76 species. We then used the resulting set of 15 biochemically characterized AKHs in a physiological assay that measuresnlipid or carbohydrate mobilization in three different lepidopteran species to learn about the functional cross-activity (receptor-ligand interactions) amongst the differentnbutterfly/moth families. Our results include novel peptide structures, demonstrate structural diversity, phylogenetic trends in peptide distribution and order-specificitynof Lepidoptera AKHs. There is almost an equal occurrence of octa-, nona-, and decapeptides, with an unparalleled emphasis on nonapeptides than in any insect order.nPrimitive species make Peram-CAH-II, an octapeptide found also in other orders, the lepidopteran signature peptide is Manse-AKH. Not all of the 15 tested AKHs are activenin Pieris brassicae, this provides insight into structure-activity specificity and could be useful for further investigations into possible biorational insecticide development.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA17-22276S" target="_blank" >GA17-22276S: Nové metody pro metabolomickou analýzu obtížně stanovitelných metabolitů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in physiology

ISSN

1664-042X

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

DEC 17

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

24

Strana od-do

614552

Kód UT WoS článku

000603644600001

EID výsledku v databázi Scopus

2-s2.0-85098643812