SubtypingCryptosporidium ryanae: A Common Pathogen in Bovine Animals

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F20%3A00538194" target="_blank" >RIV/60077344:_____/20:00538194 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2076-2607/8/8/1107" target="_blank" >https://www.mdpi.com/2076-2607/8/8/1107</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/microorganisms8081107" target="_blank" >10.3390/microorganisms8081107</a>

Jazyk výsledku

angličtina

Název v původním jazyce

SubtypingCryptosporidium ryanae: A Common Pathogen in Bovine Animals

Popis výsledku v původním jazyce

Cryptosporidium ryanaeis one of the most common species for cryptosporidiosis in cattle. However, little is known of the genetic characteristics ofC. ryanaedue to the lack of subtyping tools. In the present study, the 60-kDa glycoprotein (gp60) gene ofC. ryanaewas identified in whole genome sequence data and analyzed for sequence characteristics using bioinformatics tools. The protein it encodes had some of the typical characteristics of GP60 proteins, with a signal peptide, a furin cleavage site, and a glycosylphosphatidylinositol anchor at the C terminus of the protein, and numerous O-glycosylation sites. The gene sequence was used in the development of a subtyping tool, which was used in characterizingC. ryanaefrom 110 specimens from dairy cattle, 2 from beef cattle, 6 from yaks, and 4 from water buffaloes in China. Altogether, 17 subtypes from 8 subtype families were recognized, namely XXIa to XXIh. Possible host adaption was identified within this species, reflected by the unique occurrence of XXIa, XXIc, and XXIh in dairy cattle, yaks, and water buffaloes, respectively. Some geographical differences were detected in the distribution of subtype families in dairy cattle, specimens from southern China showed higher genetic diversity than from northern China, and the XXIa subtype family was only seen in dairy cattle in southern and eastern China. Thegp60-based subtyping tool should be useful in molecular epidemiological studies of the transmission ofC. ryanae.

Název v anglickém jazyce

SubtypingCryptosporidium ryanae: A Common Pathogen in Bovine Animals

Popis výsledku anglicky

Cryptosporidium ryanaeis one of the most common species for cryptosporidiosis in cattle. However, little is known of the genetic characteristics ofC. ryanaedue to the lack of subtyping tools. In the present study, the 60-kDa glycoprotein (gp60) gene ofC. ryanaewas identified in whole genome sequence data and analyzed for sequence characteristics using bioinformatics tools. The protein it encodes had some of the typical characteristics of GP60 proteins, with a signal peptide, a furin cleavage site, and a glycosylphosphatidylinositol anchor at the C terminus of the protein, and numerous O-glycosylation sites. The gene sequence was used in the development of a subtyping tool, which was used in characterizingC. ryanaefrom 110 specimens from dairy cattle, 2 from beef cattle, 6 from yaks, and 4 from water buffaloes in China. Altogether, 17 subtypes from 8 subtype families were recognized, namely XXIa to XXIh. Possible host adaption was identified within this species, reflected by the unique occurrence of XXIa, XXIc, and XXIh in dairy cattle, yaks, and water buffaloes, respectively. Some geographical differences were detected in the distribution of subtype families in dairy cattle, specimens from southern China showed higher genetic diversity than from northern China, and the XXIa subtype family was only seen in dairy cattle in southern and eastern China. Thegp60-based subtyping tool should be useful in molecular epidemiological studies of the transmission ofC. ryanae.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microorganisms

ISSN

2076-2607

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

1107

Kód UT WoS článku

000564827900001

EID výsledku v databázi Scopus

2-s2.0-85088567403