POS-P-2170 Cathodoluminescence imaging of cell organelles and biomineralized structures observed using cryo-scanning electron microscope

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F20%3A00541022" target="_blank" >RIV/60077344:_____/20:00541022 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081731:_____/20:00541022

Výsledek na webu

<a href="http://microscopy.cz/conferencecsms/csms-sbornik.pdf" target="_blank" >http://microscopy.cz/conferencecsms/csms-sbornik.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

POS-P-2170 Cathodoluminescence imaging of cell organelles and biomineralized structures observed using cryo-scanning electron microscope

Popis výsledku v původním jazyce

As opposed to pathogens passively circulating in the body fluids of their host, pathogenic species within the Spirochaetes phylum are able to actively coordinate their movement in the host to cause systemic infections. Based on the unique morphology and high motility of spirochetes, we hypothesized that their surface adhesive molecules might be suitably adapted to aid in their dissemination strategies. Designing a system that mimics natural environmental signals, which many spirochetes face during their infectious cycle, we observed that a subset of their surface proteins, particularly DbpA/B, can strongly enhance motility of spirochetes in the extracellular matrix of the host. Using atomic force microscopy, we disentangled the mechanistic details of DbpA/B and decorin/laminin interactions. Our results show that spirochetes are able to leverage a wide variety of adhesion strategies through force-tuning transient molecular binding to extracellular matrix components, in order to switch between different motility states to enhance its dissemination

Název v anglickém jazyce

POS-P-2170 Cathodoluminescence imaging of cell organelles and biomineralized structures observed using cryo-scanning electron microscope

Popis výsledku anglicky

As opposed to pathogens passively circulating in the body fluids of their host, pathogenic species within the Spirochaetes phylum are able to actively coordinate their movement in the host to cause systemic infections. Based on the unique morphology and high motility of spirochetes, we hypothesized that their surface adhesive molecules might be suitably adapted to aid in their dissemination strategies. Designing a system that mimics natural environmental signals, which many spirochetes face during their infectious cycle, we observed that a subset of their surface proteins, particularly DbpA/B, can strongly enhance motility of spirochetes in the extracellular matrix of the host. Using atomic force microscopy, we disentangled the mechanistic details of DbpA/B and decorin/laminin interactions. Our results show that spirochetes are able to leverage a wide variety of adhesion strategies through force-tuning transient molecular binding to extracellular matrix components, in order to switch between different motility states to enhance its dissemination

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů