Ex vivo development of Phasmarhabditis spp. associated with terrestrial molluscs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00552166" target="_blank" >RIV/60077344:_____/22:00552166 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.cambridge.org/core/journals/journal-of-helminthology/article/abs/ex-vivo-development-of-phasmarhabditis-spp-associated-with-terrestrial-molluscs/C351BC0C00D8D8E2A67754AC0368B36E" target="_blank" >https://www.cambridge.org/core/journals/journal-of-helminthology/article/abs/ex-vivo-development-of-phasmarhabditis-spp-associated-with-terrestrial-molluscs/C351BC0C00D8D8E2A67754AC0368B36E</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S0022149X21000663" target="_blank" >10.1017/S0022149X21000663</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ex vivo development of Phasmarhabditis spp. associated with terrestrial molluscs

Popis výsledku v původním jazyce

The success of Phasmarhabditis hermaphrodita (Schneider) Andrássy (Rhabditida: Rhabditidae) as a biological control agent of molluscs has led to a worldwide interest in phasmarhabditids. However, scant information is available on the lifecycle development of species within the genus. In the current study, the development of P. hermaphrodita, Phasmarhabditis papillosa, Phasmarhabditis bohemica and Phasmarhabditis kenyaensis were studied using ex vivo cultures, in order to improve our understanding of their biology. Infective juveniles (IJs) of each species were added to 1 g of defrosted homogenized slug cadavers of Deroceras invadens and the development monitored after inoculated IJ recovery, over a period of eight–ten days. The results demonstrated that P. bohemica had the shortest development cycle and that it was able to produce first-generation IJs after eight days, while P. hermaphrodita, P. papillosa and P. kenyaensis took ten days to form a new cohort of IJs. However, from the perspective of mass rearing, P. hermaphrodita has an advantage over the other species in that it is capable of forming self-fertilizing hermaphrodites, whereas both males and females are required for the reproduction of P. papillosa, P. bohemica and P. kenyaensis. The results of the study contribute to the knowledge of the biology of the genus and will help to establish the in vitro liquid cultures of different species of the genus.

Název v anglickém jazyce

Ex vivo development of Phasmarhabditis spp. associated with terrestrial molluscs

Popis výsledku anglicky

The success of Phasmarhabditis hermaphrodita (Schneider) Andrássy (Rhabditida: Rhabditidae) as a biological control agent of molluscs has led to a worldwide interest in phasmarhabditids. However, scant information is available on the lifecycle development of species within the genus. In the current study, the development of P. hermaphrodita, Phasmarhabditis papillosa, Phasmarhabditis bohemica and Phasmarhabditis kenyaensis were studied using ex vivo cultures, in order to improve our understanding of their biology. Infective juveniles (IJs) of each species were added to 1 g of defrosted homogenized slug cadavers of Deroceras invadens and the development monitored after inoculated IJ recovery, over a period of eight–ten days. The results demonstrated that P. bohemica had the shortest development cycle and that it was able to produce first-generation IJs after eight days, while P. hermaphrodita, P. papillosa and P. kenyaensis took ten days to form a new cohort of IJs. However, from the perspective of mass rearing, P. hermaphrodita has an advantage over the other species in that it is capable of forming self-fertilizing hermaphrodites, whereas both males and females are required for the reproduction of P. papillosa, P. bohemica and P. kenyaensis. The results of the study contribute to the knowledge of the biology of the genus and will help to establish the in vitro liquid cultures of different species of the genus.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10613 - Zoology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Helminthology

ISSN

0022-149X

e-ISSN

1475-2697

Svazek periodika

96

Číslo periodika v rámci svazku

JAN 11

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

e6

Kód UT WoS článku

000740996000001

EID výsledku v databázi Scopus

2-s2.0-85123562982