In vitro liquid culture of the mollusc-parasitic nematode Phasmarhabditis (Rhabditida: Rhabditidae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00564665" target="_blank" >RIV/60077344:_____/22:00564665 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.cambridge.org/core/journals/journal-of-helminthology/article/abs/in-vitro-liquid-culture-of-the-molluscparasitic-nematode-phasmarhabditis-rhabditida-rhabditidae/D515BB4F3A3A25AD308B6271E2B33D16" target="_blank" >https://www.cambridge.org/core/journals/journal-of-helminthology/article/abs/in-vitro-liquid-culture-of-the-molluscparasitic-nematode-phasmarhabditis-rhabditida-rhabditidae/D515BB4F3A3A25AD308B6271E2B33D16</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S0022149X22000736" target="_blank" >10.1017/S0022149X22000736</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vitro liquid culture of the mollusc-parasitic nematode Phasmarhabditis (Rhabditida: Rhabditidae)

Popis výsledku v původním jazyce

The success of the mollusc-parasitic nematode, Phasmarhabditis hermaphrodita (Schneider) Andrassy (Rhabditida: Rhabditidae), as a biological control agent in Europe has led to worldwide interest in phasmarhabditids as biocontrol agents. In this study, the mass culture potential of three phasmarhabditids, namely Phasmarhabditis papillosa, Phasmarhabditis kenyaensis and Phasmarhabditis bohemica, was assessed. In addition, ten bacterial candidates, consisting of seven associated with slugs and three associated with entomopathogenic nematodes, were investigated. The bacteria were tested for their ability to cause mortality to Deroceras invadens, as well as to support nematode growth. Initial mortality studies demonstrated that Kluyvera, Aeromonas and Pseudomonas spp. (AP3) caused 100% mortality when they were injected into the haemocoel of D. invadens. However, in growth studies, Pseudomonas sp. (AP4) was found to be the most successful bacterium, leading to recovery and reproduction in almost all nematode species, except for P. kenyaensis. In flask studies, P. bohemica, which showed exceptional growth with Pseudomonas sp. (AP1), was chosen for further investigation. The effect of inoculating flasks with different concentrations of Pseudomonas sp. (AP1), as well as with different concentrations of P. bohemica, was evaluated by assessing the nematode populations for 14 days. The results indicated that the lowest, 1% (v/v), bacteria inoculation led to higher total nematode and to infective juvenile (IJ) yield, with flasks with the highest IJ inoculum (3000 IJs/ml) having a positive effect on the total number of nematodes and IJs in cultures of P. bohemica. This study presents improvements for the mass-culturing of nematodes associated with molluscs.

Název v anglickém jazyce

In vitro liquid culture of the mollusc-parasitic nematode Phasmarhabditis (Rhabditida: Rhabditidae)

Popis výsledku anglicky

The success of the mollusc-parasitic nematode, Phasmarhabditis hermaphrodita (Schneider) Andrassy (Rhabditida: Rhabditidae), as a biological control agent in Europe has led to worldwide interest in phasmarhabditids as biocontrol agents. In this study, the mass culture potential of three phasmarhabditids, namely Phasmarhabditis papillosa, Phasmarhabditis kenyaensis and Phasmarhabditis bohemica, was assessed. In addition, ten bacterial candidates, consisting of seven associated with slugs and three associated with entomopathogenic nematodes, were investigated. The bacteria were tested for their ability to cause mortality to Deroceras invadens, as well as to support nematode growth. Initial mortality studies demonstrated that Kluyvera, Aeromonas and Pseudomonas spp. (AP3) caused 100% mortality when they were injected into the haemocoel of D. invadens. However, in growth studies, Pseudomonas sp. (AP4) was found to be the most successful bacterium, leading to recovery and reproduction in almost all nematode species, except for P. kenyaensis. In flask studies, P. bohemica, which showed exceptional growth with Pseudomonas sp. (AP1), was chosen for further investigation. The effect of inoculating flasks with different concentrations of Pseudomonas sp. (AP1), as well as with different concentrations of P. bohemica, was evaluated by assessing the nematode populations for 14 days. The results indicated that the lowest, 1% (v/v), bacteria inoculation led to higher total nematode and to infective juvenile (IJ) yield, with flasks with the highest IJ inoculum (3000 IJs/ml) having a positive effect on the total number of nematodes and IJs in cultures of P. bohemica. This study presents improvements for the mass-culturing of nematodes associated with molluscs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Helminthology

ISSN

0022-149X

e-ISSN

1475-2697

Svazek periodika

96

Číslo periodika v rámci svazku

NOV 15

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

e84

Kód UT WoS článku

000883327500001

EID výsledku v databázi Scopus

2-s2.0-85141967590