The exact timing of microinjection of parthenogenetic silkworm embryos is crucial for their successful transgenesis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00556043" target="_blank" >RIV/60077344:_____/22:00556043 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/22:43904985

Výsledek na webu

<a href="https://www.frontiersin.org/articles/10.3389/fphys.2022.822900/pdf" target="_blank" >https://www.frontiersin.org/articles/10.3389/fphys.2022.822900/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fphys.2022.822900" target="_blank" >10.3389/fphys.2022.822900</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The exact timing of microinjection of parthenogenetic silkworm embryos is crucial for their successful transgenesis

Popis výsledku v původním jazyce

The use of parthenogenetic silkworm (Bombyx mori) strains, which eliminate the problem of recombination, is a useful tool for maintaining transgenic clonal lines. The generation of genetically identical individuals is becoming an important tool in genetic engineering, allowing replication of an existing advantageous trait combination without the mixing that occurs during sexual reproduction. Thus, an animal with a particular genetic modification, such as the ability to produce transgenic proteins, can reproduce more rapidly than by natural mating. One obstacle to the widespread use of parthenogenesis in silkworm genetic engineering is the relatively low efficiency of downstream transgenesis techniques. In this work, we seek to optimize the use of transgenesis in conjunction with the production of parthenogenetic individuals. We found that a very important parameter for the introduction of foreign genes into anparthenogenetic strain is the precise timing of embryo microinjection. Our modification of the original method increased the efficiency of transgene injection as well as the survivalnrate of injected embryos. We also provide a detailed description of the methodological procedure including a graphical overview of the entire protocol.

Název v anglickém jazyce

The exact timing of microinjection of parthenogenetic silkworm embryos is crucial for their successful transgenesis

Popis výsledku anglicky

The use of parthenogenetic silkworm (Bombyx mori) strains, which eliminate the problem of recombination, is a useful tool for maintaining transgenic clonal lines. The generation of genetically identical individuals is becoming an important tool in genetic engineering, allowing replication of an existing advantageous trait combination without the mixing that occurs during sexual reproduction. Thus, an animal with a particular genetic modification, such as the ability to produce transgenic proteins, can reproduce more rapidly than by natural mating. One obstacle to the widespread use of parthenogenesis in silkworm genetic engineering is the relatively low efficiency of downstream transgenesis techniques. In this work, we seek to optimize the use of transgenesis in conjunction with the production of parthenogenetic individuals. We found that a very important parameter for the introduction of foreign genes into anparthenogenetic strain is the precise timing of embryo microinjection. Our modification of the original method increased the efficiency of transgene injection as well as the survivalnrate of injected embryos. We also provide a detailed description of the methodological procedure including a graphical overview of the entire protocol.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Physiology

ISSN

1664-042X

e-ISSN

1664-042X

Svazek periodika

13

Číslo periodika v rámci svazku

MAR 25

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

10

Strana od-do

822900

Kód UT WoS článku

000792803400001

EID výsledku v databázi Scopus

2-s2.0-85128352651