Molecular evidence of Monocercomonas and Acanthamoeba in the feces of captive reptiles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00564291" target="_blank" >RIV/60077344:_____/22:00564291 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/22:10457209

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s00436-022-07677-3" target="_blank" >https://link.springer.com/article/10.1007/s00436-022-07677-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00436-022-07677-3" target="_blank" >10.1007/s00436-022-07677-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular evidence of Monocercomonas and Acanthamoeba in the feces of captive reptiles

Popis výsledku v původním jazyce

Reptiles are frequently kept as pet animals. They are considered as important reservoirs of protozoa with veterinary-medical significance. At a reptile farm in Ireland, fecal samples were collected from 98 captive reptiles, representing 43 species of three orders (Squamata, Testudines, and Crocodylia). After DNA extraction, all samples were screened by conventional PCRs, targeting the ribosomal small subunit (SSU) RNA and alpha-tubulin genes of trichomonads and SSU RNA gene of Acanthamoeba spp. One leopard gecko (Eublepharis macularius) was positive for a not yet reported species/genotype of the genus Monocercomonas, different from M. colubrorum. Various Acanthamoeba genotypes were detected in six reptilian species, i.e., Acanthamoeba genotype T11 in Eunectes notaeus and Heloderma suspectum/horridum, genotype T4 in Varanus exanthematicus, Chlamydosaurus kingii, and Macrochelys temminckii, and the genotype T13 in Iguana iguana. Some of these amoeba species might have clinicopathological significance in both humans and animals. Our findings highlight the importance to monitor pathogenic protozoa in pet as well as wildlife reptiles, as a source of possible infection for animals and humans living nearby.

Název v anglickém jazyce

Molecular evidence of Monocercomonas and Acanthamoeba in the feces of captive reptiles

Popis výsledku anglicky

Reptiles are frequently kept as pet animals. They are considered as important reservoirs of protozoa with veterinary-medical significance. At a reptile farm in Ireland, fecal samples were collected from 98 captive reptiles, representing 43 species of three orders (Squamata, Testudines, and Crocodylia). After DNA extraction, all samples were screened by conventional PCRs, targeting the ribosomal small subunit (SSU) RNA and alpha-tubulin genes of trichomonads and SSU RNA gene of Acanthamoeba spp. One leopard gecko (Eublepharis macularius) was positive for a not yet reported species/genotype of the genus Monocercomonas, different from M. colubrorum. Various Acanthamoeba genotypes were detected in six reptilian species, i.e., Acanthamoeba genotype T11 in Eunectes notaeus and Heloderma suspectum/horridum, genotype T4 in Varanus exanthematicus, Chlamydosaurus kingii, and Macrochelys temminckii, and the genotype T13 in Iguana iguana. Some of these amoeba species might have clinicopathological significance in both humans and animals. Our findings highlight the importance to monitor pathogenic protozoa in pet as well as wildlife reptiles, as a source of possible infection for animals and humans living nearby.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasitology Research

ISSN

0932-0113

e-ISSN

1432-1955

Svazek periodika

121

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

3681-3687

Kód UT WoS článku

000862747600001

EID výsledku v databázi Scopus

2-s2.0-85139131979