Collective behavior of magnetic microrobots through immuno-sandwich assay: On-the-fly COVID-19 sensing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00568185" target="_blank" >RIV/60077344:_____/22:00568185 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22310/22:43924077 RIV/00027162:_____/22:N0000009 RIV/60461373:22350/22:43924077 RIV/60076658:12310/22:43905040 RIV/62156489:43210/22:43920985

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2352940721003875?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2352940721003875?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.apmt.2021.101337" target="_blank" >10.1016/j.apmt.2021.101337</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Collective behavior of magnetic microrobots through immuno-sandwich assay: On-the-fly COVID-19 sensing

Popis výsledku v původním jazyce

Mobile self-propelled micro/nanorobots are mobile binding surface that improved the sensitivity of many biosensing system by on-the-fly identification and isolation of different biotargets. Proteins are powerful tools to predict infectious disease progression such as COVID-19. The main methodology used to COVID-19 detection is based on ELISA test by antibodies detection assays targeting SARS-CoV-2 virus spike protein and nucleocapside protein that represent an indirect SARS-CoV-2 detection with low sentitivy and specificity. Moreover ELISA test are limited to used external shaker to obtain homogenously immobilization of antibodies and protein on sensing platform. Here, we present magnetic microrobots that collective self-assembly through immuno-sandwich assay and they can be used as mobile platform to detect on-the-fly SARS-CoV-2 virus particle by its spike protein. The collective self-assembly of magnetic microrobots through immuno-sandwich assay enhanced its analytical performance in terms of sensitivity decreasing the detection limit of SARS-CoV-2 virus by one order of magnitude with respect to the devices previously reported. This proof-of-concept of microrobotics offer new ways to the detection of viruses and proteins of medical interest in general.(c) 2021 Elsevier Ltd. All rights reserved.

Název v anglickém jazyce

Collective behavior of magnetic microrobots through immuno-sandwich assay: On-the-fly COVID-19 sensing

Popis výsledku anglicky

Mobile self-propelled micro/nanorobots are mobile binding surface that improved the sensitivity of many biosensing system by on-the-fly identification and isolation of different biotargets. Proteins are powerful tools to predict infectious disease progression such as COVID-19. The main methodology used to COVID-19 detection is based on ELISA test by antibodies detection assays targeting SARS-CoV-2 virus spike protein and nucleocapside protein that represent an indirect SARS-CoV-2 detection with low sentitivy and specificity. Moreover ELISA test are limited to used external shaker to obtain homogenously immobilization of antibodies and protein on sensing platform. Here, we present magnetic microrobots that collective self-assembly through immuno-sandwich assay and they can be used as mobile platform to detect on-the-fly SARS-CoV-2 virus particle by its spike protein. The collective self-assembly of magnetic microrobots through immuno-sandwich assay enhanced its analytical performance in terms of sensitivity decreasing the detection limit of SARS-CoV-2 virus by one order of magnitude with respect to the devices previously reported. This proof-of-concept of microrobotics offer new ways to the detection of viruses and proteins of medical interest in general.(c) 2021 Elsevier Ltd. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20503 - Textiles; including synthetic dyes, colours, fibres (nanoscale materials to be 2.10; biomaterials to be 2.9)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Materials Today

ISSN

2352-9407

e-ISSN

2352-9407

Svazek periodika

26

Číslo periodika v rámci svazku

MAR

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

15

Strana od-do

101337

Kód UT WoS článku

000784043100001

EID výsledku v databázi Scopus

2-s2.0-85122311148