Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00583894" target="_blank" >RIV/60077344:_____/23:00583894 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/1422-0067/24/20/15350" target="_blank" >https://www.mdpi.com/1422-0067/24/20/15350</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms242015350" target="_blank" >10.3390/ijms242015350</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties

Popis výsledku v původním jazyce

Three basil plant varieties (Ocimum basilicum var. Genovese, Ocimum x citriodorum, and Ocimum basilicum var. purpurascens) were grown under moderate light (about 300 mu mol photons m(-2) s(-1)) in a glasshouse or growth chamber and then either transferred to an open field (average daily dose: 29.2 kJ m(-2) d-1) or additionally exposed to UV-B irradiation in a growth chamber (29.16 kJ m(-2) d(-1)), to reveal the variety-specific and light-specific acclimation responses. Total antioxidant capacity (TAC), phenolic profile, ascorbate content, and class III peroxidase (POD) activity were used to determine the antioxidant status of leaves under all four light regimes. Exposure to high solar irradiation at the open field resulted in an increase in TAC, total hydroxycinnamic acids (HCAs, especially caffeic acid), flavonoids, and epidermal UV-absorbing substances in all three varieties, as well as a two-fold increase in the leaf dry/fresh weight ratio. The supplemental UV-B irradiation induced preferential accumulation of HCAs (rosmarinic acid) over flavonoids, increased TAC and POD activity, but decreased the ascorbate content in the leaves, and inhibited the accumulation of epidermal flavonoids in all basil varieties. Furthermore, characteristic leaf curling and UV-B-induced inhibition of plant growth were observed in all basil varieties, while a pro-oxidant effect of UV-B was indicated with H2O2 accumulation in the leaves and spotty leaf browning. The extent of these morphological changes, and oxidative damage depended on the basil cultivar, implies a genotype-specific tolerance mechanism to high doses of UV-B irradiation.

Název v anglickém jazyce

Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties

Popis výsledku anglicky

Three basil plant varieties (Ocimum basilicum var. Genovese, Ocimum x citriodorum, and Ocimum basilicum var. purpurascens) were grown under moderate light (about 300 mu mol photons m(-2) s(-1)) in a glasshouse or growth chamber and then either transferred to an open field (average daily dose: 29.2 kJ m(-2) d-1) or additionally exposed to UV-B irradiation in a growth chamber (29.16 kJ m(-2) d(-1)), to reveal the variety-specific and light-specific acclimation responses. Total antioxidant capacity (TAC), phenolic profile, ascorbate content, and class III peroxidase (POD) activity were used to determine the antioxidant status of leaves under all four light regimes. Exposure to high solar irradiation at the open field resulted in an increase in TAC, total hydroxycinnamic acids (HCAs, especially caffeic acid), flavonoids, and epidermal UV-absorbing substances in all three varieties, as well as a two-fold increase in the leaf dry/fresh weight ratio. The supplemental UV-B irradiation induced preferential accumulation of HCAs (rosmarinic acid) over flavonoids, increased TAC and POD activity, but decreased the ascorbate content in the leaves, and inhibited the accumulation of epidermal flavonoids in all basil varieties. Furthermore, characteristic leaf curling and UV-B-induced inhibition of plant growth were observed in all basil varieties, while a pro-oxidant effect of UV-B was indicated with H2O2 accumulation in the leaves and spotty leaf browning. The extent of these morphological changes, and oxidative damage depended on the basil cultivar, implies a genotype-specific tolerance mechanism to high doses of UV-B irradiation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/EF15_003%2F0000336" target="_blank" >EF15_003/0000336: Kovy, rostliny a lidé</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Molecular Sciences

ISSN

1661-6596

e-ISSN

—

Svazek periodika

24

Číslo periodika v rámci svazku

20

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

18

Strana od-do

15350

Kód UT WoS článku

001099423700001

EID výsledku v databázi Scopus

2-s2.0-85175277079