Disruption of the standard kinetochore in holocentric Cuscuta species
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00584248" target="_blank" >RIV/60077344:_____/23:00584248 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.pnas.org/doi/10.1073/pnas.2300877120" target="_blank" >https://www.pnas.org/doi/10.1073/pnas.2300877120</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1073/pnas.2300877120" target="_blank" >10.1073/pnas.2300877120</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Disruption of the standard kinetochore in holocentric Cuscuta species
Popis výsledku v původním jazyce
The segregation of chromosomes depends on the centromere. Most species are monocentric, with the centromere restricted to a single region per chromosome. In some organisms, the monocentric organization changed to holocentric, in which the centromere activity is distributed over the entire chromosome length. However, the causes and consequences of this transition are poorly understood. Here, we show that the transition in the genus Cuscuta was associated with dramatic changes in the kinetochore, a protein complex that mediates the attachment of chromosomes to microtubules. We found that in holocentric Cuscuta species, the KNL2 genes were lost, the CENP-C, KNL1, and ZWINT1 genes were truncated, the centromeric localization of CENH3, CENP-C, KNL1, MIS12, and NDC80 proteins was disrupted, and the spindle assembly checkpoint (SAC) degenerated. Our results demonstrate that holocentric Cuscuta species lost the ability to form a standard kinetochore and do not employ SAC to control the attachment of microtubules to chromosomes.
Název v anglickém jazyce
Disruption of the standard kinetochore in holocentric Cuscuta species
Popis výsledku anglicky
The segregation of chromosomes depends on the centromere. Most species are monocentric, with the centromere restricted to a single region per chromosome. In some organisms, the monocentric organization changed to holocentric, in which the centromere activity is distributed over the entire chromosome length. However, the causes and consequences of this transition are poorly understood. Here, we show that the transition in the genus Cuscuta was associated with dramatic changes in the kinetochore, a protein complex that mediates the attachment of chromosomes to microtubules. We found that in holocentric Cuscuta species, the KNL2 genes were lost, the CENP-C, KNL1, and ZWINT1 genes were truncated, the centromeric localization of CENH3, CENP-C, KNL1, MIS12, and NDC80 proteins was disrupted, and the spindle assembly checkpoint (SAC) degenerated. Our results demonstrate that holocentric Cuscuta species lost the ability to form a standard kinetochore and do not employ SAC to control the attachment of microtubules to chromosomes.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10603 - Genetics and heredity (medical genetics to be 3)
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
e-ISSN
—
Svazek periodika
120
Číslo periodika v rámci svazku
21
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
10
Strana od-do
e2300877120
Kód UT WoS článku
001039496800005
EID výsledku v databázi Scopus
2-s2.0-85161349225