Juvenile hormones direct primordial germ cell migration to the embryonic gonad

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00584656" target="_blank" >RIV/60077344:_____/24:00584656 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.cub.2023.12.033" target="_blank" >https://doi.org/10.1016/j.cub.2023.12.033</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cub.2023.12.033" target="_blank" >10.1016/j.cub.2023.12.033</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Juvenile hormones direct primordial germ cell migration to the embryonic gonad

Popis výsledku v původním jazyce

Germ cells are essential to sexual reproduction. Across the animal kingdom, extracellular signaling isoprenoids, such as retinoic acids (RAs) in vertebrates and juvenile hormones (JHs) in invertebrates, facilitate multiple processes in reproduction. Here we investigated the role of these potent signaling molecules in embryonic germ cell development, using JHs in Drosophila melanogaster as a model system. In contrast to their established endocrine roles during larval and adult germline development, we found that JH signaling acts locally during embryonic development. Using an in vivo biosensor, we observed active JH signaling first within and near primordial germ cells (PGCs) as they migrate to the developing gonad. Through in vivo and in vitro assays, we determined that JHs are both necessary and sufficient for PGC migration. Analysis into the mechanisms of this newly uncovered paracrine JH function revealed that PGC migration was compromised when JHs were decreased or increased, suggesting that specific titers or spatiotemporal JH dynamics are required for robust PGC colonization of the gonad. Compromised PGC migration can impair fertility and cause germ cell tumors in many species, including humans. In mammals, retinoids have many roles in development and reproduction. We found that like JHs in Drosophila, RA was sufficient to impact mouse PGC migration in vitro. Together, our study reveals a previously unanticipated role of isoprenoids as local effectors of pregonadal PGC development and suggests a broadly shared mechanism in PGC migration.

Název v anglickém jazyce

Juvenile hormones direct primordial germ cell migration to the embryonic gonad

Popis výsledku anglicky

Germ cells are essential to sexual reproduction. Across the animal kingdom, extracellular signaling isoprenoids, such as retinoic acids (RAs) in vertebrates and juvenile hormones (JHs) in invertebrates, facilitate multiple processes in reproduction. Here we investigated the role of these potent signaling molecules in embryonic germ cell development, using JHs in Drosophila melanogaster as a model system. In contrast to their established endocrine roles during larval and adult germline development, we found that JH signaling acts locally during embryonic development. Using an in vivo biosensor, we observed active JH signaling first within and near primordial germ cells (PGCs) as they migrate to the developing gonad. Through in vivo and in vitro assays, we determined that JHs are both necessary and sufficient for PGC migration. Analysis into the mechanisms of this newly uncovered paracrine JH function revealed that PGC migration was compromised when JHs were decreased or increased, suggesting that specific titers or spatiotemporal JH dynamics are required for robust PGC colonization of the gonad. Compromised PGC migration can impair fertility and cause germ cell tumors in many species, including humans. In mammals, retinoids have many roles in development and reproduction. We found that like JHs in Drosophila, RA was sufficient to impact mouse PGC migration in vitro. Together, our study reveals a previously unanticipated role of isoprenoids as local effectors of pregonadal PGC development and suggests a broadly shared mechanism in PGC migration.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA22-21244S" target="_blank" >GA22-21244S: Výzkum regulace vývoje a reprodukce řízené juvenilním hormonem (JH) u komárů, s využitím mutantů, kteří neprodukují JH</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Current Biology

ISSN

0960-9822

e-ISSN

1879-0445

Svazek periodika

34

Číslo periodika v rámci svazku

FEB 5 2024

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

21

Strana od-do

č. 3

Kód UT WoS článku

001181652400001

EID výsledku v databázi Scopus

2-s2.0-85183020212