Low potential of fish as a source of infection with Angiostrongylus cantonensis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00601319" target="_blank" >RIV/60077344:_____/24:00601319 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/24:00137227 RIV/60460709:41210/24:98614 RIV/62157124:16170/24:43881509

Výsledek na webu

<a href="https://doi.org/10.1093/fqsafe/fyae042" target="_blank" >https://doi.org/10.1093/fqsafe/fyae042</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/fqsafe/fyae042" target="_blank" >10.1093/fqsafe/fyae042</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Low potential of fish as a source of infection with Angiostrongylus cantonensis

Popis výsledku v původním jazyce

Objectives: Fish are hypothesized to act as paratenic hosts for the zoonotic nematode Angiostrongylus cantonensis, which causes human eosinophilic meningitis.There is a lack of data confirming the relevance of fish in A. cantonensis life cycle and their contribution to human infection. Materials and Methods: We conducted a series of experiments to investigate the survival and infectivity of A. cantonensis larvae in Clarias gariepinus (catfish n =30) and Oreochromis niloticus (tilapia n =24). Each fish was inoculated with 10 000 third-stage larvae (L3). Larval survival was assessed through artificial digestion of fish tissues 1 week, 2 weeks, and 3 weeks post-infection. To investigate the early stages of infection, four catfish were inoculated with 10 000 L3 each and sacrificed 3 d post-infection. Quantitative polymerase chain reaction (qPCR) analysis and histopathological examination were performed to evaluate larval distribution and tissue reactions. Two infected catfish, sacrificed 1 week post-infection, were used to feed Wistar rats. Results: After 45 d, the rats did not shed first-stage larvae (L1), indicating the absence of infection. One week post-infection, dead larvae were present in the digested tissues of both fish species, and the same was observed 2 weeks and 3 weeks after exposure. qPCR analysis revealed that the intestine was the most heavily infected organ. Histopathological examination identified dead larvae within granulomas in both the testines and liver. Early-stage infection experiment showed that fish sacrificed 3 d post-inoculation contained viable L3, which was infective Wistar rats. Conclusions: While A. cantonensis L3 can survive and remain infective in fish for a short period, they typically die within first few days post infection. This suggests that fish may not be significant long-term paratenic hosts for A. cantonensis but may play a temporary role in its transmission to mammals (including humans) and birds. These results are consistent with previous studies on freshwater shrimps and highlight the importance of understanding aquatic host interactions in the epidemiology of this zoonotic foodborne pathogen.

Název v anglickém jazyce

Low potential of fish as a source of infection with Angiostrongylus cantonensis

Popis výsledku anglicky

Objectives: Fish are hypothesized to act as paratenic hosts for the zoonotic nematode Angiostrongylus cantonensis, which causes human eosinophilic meningitis.There is a lack of data confirming the relevance of fish in A. cantonensis life cycle and their contribution to human infection. Materials and Methods: We conducted a series of experiments to investigate the survival and infectivity of A. cantonensis larvae in Clarias gariepinus (catfish n =30) and Oreochromis niloticus (tilapia n =24). Each fish was inoculated with 10 000 third-stage larvae (L3). Larval survival was assessed through artificial digestion of fish tissues 1 week, 2 weeks, and 3 weeks post-infection. To investigate the early stages of infection, four catfish were inoculated with 10 000 L3 each and sacrificed 3 d post-infection. Quantitative polymerase chain reaction (qPCR) analysis and histopathological examination were performed to evaluate larval distribution and tissue reactions. Two infected catfish, sacrificed 1 week post-infection, were used to feed Wistar rats. Results: After 45 d, the rats did not shed first-stage larvae (L1), indicating the absence of infection. One week post-infection, dead larvae were present in the digested tissues of both fish species, and the same was observed 2 weeks and 3 weeks after exposure. qPCR analysis revealed that the intestine was the most heavily infected organ. Histopathological examination identified dead larvae within granulomas in both the testines and liver. Early-stage infection experiment showed that fish sacrificed 3 d post-inoculation contained viable L3, which was infective Wistar rats. Conclusions: While A. cantonensis L3 can survive and remain infective in fish for a short period, they typically die within first few days post infection. This suggests that fish may not be significant long-term paratenic hosts for A. cantonensis but may play a temporary role in its transmission to mammals (including humans) and birds. These results are consistent with previous studies on freshwater shrimps and highlight the importance of understanding aquatic host interactions in the epidemiology of this zoonotic foodborne pathogen.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/GA22-26136S" target="_blank" >GA22-26136S: Probíhající globální invaze zoonotické hlístice Angiostrongylus cantonensis: analýza rizik rozšíření v Evropě</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Quality and Safety

ISSN

2399-1399

e-ISSN

2399-1402

Svazek periodika

8

Číslo periodika v rámci svazku

NOV

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

fyae042

Kód UT WoS článku

001350931200001

EID výsledku v databázi Scopus

2-s2.0-85211139497