Cryopreservation of strawberry (Fragaria x ananassa) germplasm and two aphid-transmitted strawberry viruses in Norway

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00601756" target="_blank" >RIV/60077344:_____/24:00601756 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.pubhort.org/ejhs/89/3/15/index.htm" target="_blank" >https://www.pubhort.org/ejhs/89/3/15/index.htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/eJHS.2024/015" target="_blank" >10.17660/eJHS.2024/015</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cryopreservation of strawberry (Fragaria x ananassa) germplasm and two aphid-transmitted strawberry viruses in Norway

Popis výsledku v původním jazyce

Background of the study- Cryopreservation is considered to be a valuable method for long-term preservation of plant germplasm and recently it has been shown to be a reliable method for preserving obligate pathogens including plant viruses. Objectives- (1) Droplet-vitrification cryopreservation of strawberry genotypes in Norway, (2) Preservation efficiency of aphid-transmitted strawberry mild yellow edge virus (SMYEV) and strawberry vein banding virus (SVBV) following cryopreservation. Methods- Excised shoot tips of cv. Korona were cryopreserved with different durations of PVS2 varying from 10 to 60 min, whereas virus-infected shoot tips were cryopreserved using either 10, 40 or 60 min of PVS2. Results- The results showed that 40-60 minutes of PVS2 treatment was more efficient for preserving strawberry germplasm than lower duration times (10-30 min). Thirtytwo strawberry genotypes have been successfully cryopreserved through droplet-vitrification with regeneration rates ranging from 45% to 100% with 40 min PVS2 treatment. Cryopreserved viruses were quantitatively analyzed by Reverse Transcription-quantitative polymerase chain reaction (RT-qPCR). SVBV was successfully cryopreserved in all the regenerated shoots following cryopreservation with all the three durations of PVS2 examined. SMYEV, however, was more efficiently preserved in shoot tips exposed to 40 min (90%) of PVS2, in comparison to 60 min (33%). Conclusion-This demonstrates that SMYEV and SVBV can be successfully cryopreserved in living cells of Fragaria ssp. by droplet vitrification. The results indicate that cryopreservation has great potential for long-time preservation of both strawberry germplasm and aphid-transmitted strawberry-infecting viruses.

Název v anglickém jazyce

Cryopreservation of strawberry (Fragaria x ananassa) germplasm and two aphid-transmitted strawberry viruses in Norway

Popis výsledku anglicky

Background of the study- Cryopreservation is considered to be a valuable method for long-term preservation of plant germplasm and recently it has been shown to be a reliable method for preserving obligate pathogens including plant viruses. Objectives- (1) Droplet-vitrification cryopreservation of strawberry genotypes in Norway, (2) Preservation efficiency of aphid-transmitted strawberry mild yellow edge virus (SMYEV) and strawberry vein banding virus (SVBV) following cryopreservation. Methods- Excised shoot tips of cv. Korona were cryopreserved with different durations of PVS2 varying from 10 to 60 min, whereas virus-infected shoot tips were cryopreserved using either 10, 40 or 60 min of PVS2. Results- The results showed that 40-60 minutes of PVS2 treatment was more efficient for preserving strawberry germplasm than lower duration times (10-30 min). Thirtytwo strawberry genotypes have been successfully cryopreserved through droplet-vitrification with regeneration rates ranging from 45% to 100% with 40 min PVS2 treatment. Cryopreserved viruses were quantitatively analyzed by Reverse Transcription-quantitative polymerase chain reaction (RT-qPCR). SVBV was successfully cryopreserved in all the regenerated shoots following cryopreservation with all the three durations of PVS2 examined. SMYEV, however, was more efficiently preserved in shoot tips exposed to 40 min (90%) of PVS2, in comparison to 60 min (33%). Conclusion-This demonstrates that SMYEV and SVBV can be successfully cryopreserved in living cells of Fragaria ssp. by droplet vitrification. The results indicate that cryopreservation has great potential for long-time preservation of both strawberry germplasm and aphid-transmitted strawberry-infecting viruses.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/TO01000295" target="_blank" >TO01000295: Zdravé ovoce v měnících se klimatických podmínkách: vývoj nových biotechnologických postupů diagnostiky virů, studium vektorů, ozdravování a bezpečného uchovávání jahodníku a maliníku</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Horticultural Scienc

ISSN

1611-4426

e-ISSN

1611-4434

Svazek periodika

89

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

BE - Belgické království

Počet stran výsledku

8

Strana od-do

1-8

Kód UT WoS článku

001332714700001

EID výsledku v databázi Scopus

2-s2.0-85210445031