Role of Aralkylamine N‐Acetyltransferase in the Response to Antioxidative Stress in the Fruit Fly Drosophila Melanogaster Adults

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00601765" target="_blank" >RIV/60077344:_____/24:00601765 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/24:43908474 RIV/60076658:12520/24:43908474

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1002/arch.70009" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1002/arch.70009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/arch.70009" target="_blank" >10.1002/arch.70009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Role of Aralkylamine N‐Acetyltransferase in the Response to Antioxidative Stress in the Fruit Fly Drosophila Melanogaster Adults

Popis výsledku v původním jazyce

In multicellular organisms, the indole melatonin synthesized by aralkylamine N‐acetyltransferase (AANATI) serves as an antioxidant. To test this, sex‐mixed 3‐day‐old mated fly adults bw1 and AANAT1 homozygous recessive loss‐of‐function mutant (bw AANAT1lo) of Drosophila melanogaster were fed by a standard diet or by one containing paraquat (PQ, 1,1′‐dimethyl‐4,4′‐bipyridilium dichloride hydrate) at a final concentration of 15.5 mM. Experiment lasted 8 h and began at 11 a.m. In bw1 flies the paraquat treatment resulted in a significant (evaluated by Student's t‐tests) decrease of the superoxide dismutase (SOD) activity and an increase the catalase (CAT) and glutathione S‐transferase (GST) activities. Meanwhile, in these flies, total Antioxidative capacity (TAC) was significantly curbed by the paraquat presence. Importantly, these changes were not observed in the AANAT1‐mutants. Thus, melatonin seems to play an important defence role against the oxidative stress elicited by paraquat.

Název v anglickém jazyce

Role of Aralkylamine N‐Acetyltransferase in the Response to Antioxidative Stress in the Fruit Fly Drosophila Melanogaster Adults

Popis výsledku anglicky

In multicellular organisms, the indole melatonin synthesized by aralkylamine N‐acetyltransferase (AANATI) serves as an antioxidant. To test this, sex‐mixed 3‐day‐old mated fly adults bw1 and AANAT1 homozygous recessive loss‐of‐function mutant (bw AANAT1lo) of Drosophila melanogaster were fed by a standard diet or by one containing paraquat (PQ, 1,1′‐dimethyl‐4,4′‐bipyridilium dichloride hydrate) at a final concentration of 15.5 mM. Experiment lasted 8 h and began at 11 a.m. In bw1 flies the paraquat treatment resulted in a significant (evaluated by Student's t‐tests) decrease of the superoxide dismutase (SOD) activity and an increase the catalase (CAT) and glutathione S‐transferase (GST) activities. Meanwhile, in these flies, total Antioxidative capacity (TAC) was significantly curbed by the paraquat presence. Importantly, these changes were not observed in the AANAT1‐mutants. Thus, melatonin seems to play an important defence role against the oxidative stress elicited by paraquat.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10605 - Developmental biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Archives of Insect Biochemistry and Physiology

ISSN

0739-4462

e-ISSN

1520-6327

Svazek periodika

117

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

e70009

Kód UT WoS článku

001370235600001

EID výsledku v databázi Scopus

2-s2.0-85210085627