Truncating the spliceosomal 'rope protein' Prp45 results in Htz1 dependent phenotypes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00603215" target="_blank" >RIV/60077344:_____/24:00603215 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/24:10487127

Výsledek na webu

<a href="https://doi.org/10.1080/15476286.2024.2348896" target="_blank" >https://doi.org/10.1080/15476286.2024.2348896</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/15476286.2024.2348896" target="_blank" >10.1080/15476286.2024.2348896</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Truncating the spliceosomal 'rope protein' Prp45 results in Htz1 dependent phenotypes

Popis výsledku v původním jazyce

Spliceosome assembly contributes an important but incompletely understood aspect of splicing regulation. Prp45 is a yeast splicing factor which runs as an extended fold through the spliceosome, and which may be important for bringing its components together. We performed a whole genome analysis of the genetic interaction network of the truncated allele of PRP45 (prp45(1-169)) using synthetic genetic array technology and found chromatin remodellers and modifiers as an enriched category. In agreement with related studies, H2A.Z-encoding HTZ1, and the components of SWR1, INO80, and SAGA complexes represented prominent interactors, with htz1 conferring the strongest growth defect. Because the truncation of Prp45 disproportionately affected low copy number transcripts of intron-containing genes, we prepared strains carrying intronless versions of SRB2, VPS75, or HRB1, the most affected cases with transcription-related function. Intron removal from SRB2, but not from the other genes, partly repaired some but not all the growth phenotypes identified in the genetic screen. The interaction of prp45(1-169) and htz1 Delta was detectable even in cells with SRB2 intron deleted (srb2 Delta i). The less truncated variant, prp45(1-330), had a synthetic growth defect with htz1 Delta at 16 degrees C, which also persisted in the srb2 Delta i background. Moreover, htz1 Delta enhanced prp45(1-330) dependent pre-mRNA hyper-accumulation of both high and low efficiency splicers, genes ECM33 and COF1, respectively. We conclude that while the expression defects of low expression intron-containing genes contribute to the genetic interactome of prp45(1-169), the genetic interactions between prp45 and htz1 alleles demonstrate the sensitivity of spliceosome assembly, delayed in prp45(1-169), to the chromatin environment.

Název v anglickém jazyce

Truncating the spliceosomal 'rope protein' Prp45 results in Htz1 dependent phenotypes

Popis výsledku anglicky

Spliceosome assembly contributes an important but incompletely understood aspect of splicing regulation. Prp45 is a yeast splicing factor which runs as an extended fold through the spliceosome, and which may be important for bringing its components together. We performed a whole genome analysis of the genetic interaction network of the truncated allele of PRP45 (prp45(1-169)) using synthetic genetic array technology and found chromatin remodellers and modifiers as an enriched category. In agreement with related studies, H2A.Z-encoding HTZ1, and the components of SWR1, INO80, and SAGA complexes represented prominent interactors, with htz1 conferring the strongest growth defect. Because the truncation of Prp45 disproportionately affected low copy number transcripts of intron-containing genes, we prepared strains carrying intronless versions of SRB2, VPS75, or HRB1, the most affected cases with transcription-related function. Intron removal from SRB2, but not from the other genes, partly repaired some but not all the growth phenotypes identified in the genetic screen. The interaction of prp45(1-169) and htz1 Delta was detectable even in cells with SRB2 intron deleted (srb2 Delta i). The less truncated variant, prp45(1-330), had a synthetic growth defect with htz1 Delta at 16 degrees C, which also persisted in the srb2 Delta i background. Moreover, htz1 Delta enhanced prp45(1-330) dependent pre-mRNA hyper-accumulation of both high and low efficiency splicers, genes ECM33 and COF1, respectively. We conclude that while the expression defects of low expression intron-containing genes contribute to the genetic interactome of prp45(1-169), the genetic interactions between prp45 and htz1 alleles demonstrate the sensitivity of spliceosome assembly, delayed in prp45(1-169), to the chromatin environment.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

RNA biology

ISSN

1547-6286

e-ISSN

1555-8584

Svazek periodika

21

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

18

Strana od-do

543-559

Kód UT WoS článku

001237152800001

EID výsledku v databázi Scopus

2-s2.0-85192166077