Incidence of aphid-transmitted viruses in raspberry and raspberry aphids in Norway and experiments on aphid transmission of black raspberry necrosis virus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00604535" target="_blank" >RIV/60077344:_____/24:00604535 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1441145/full" target="_blank" >https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1441145/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2024.1441145" target="_blank" >10.3389/fpls.2024.1441145</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Incidence of aphid-transmitted viruses in raspberry and raspberry aphids in Norway and experiments on aphid transmission of black raspberry necrosis virus

Popis výsledku v původním jazyce

Raspberry (Rubus idaeus L.) is susceptible to aphid-borne viruses. We studied the incidence of four of them black raspberry necrosis virus (BRNV), raspberry leaf mottle virus (RLMV), raspberry vein chlorosis virus (RVCV), and Rubus yellow net virus (RYNV) in raspberry plants and aphids in and around Norwegian raspberry crops for three years (2019, 2021, and 2022). Most of the samples were from symptomatic plants. Applying RT-PCR, 274 leaf samples and 107 aphid samples were analyzed. All four viruses were found, but BRNV dominated: it was detected in 93% of the 178 leaf samples with virus and was the only virus that occurred more frequently as a single infection than in co-infections with the other viruses. The old cv. Veten had the highest virus incidence (97%) among the sampled plants, followed by uncultivated raspberry in the boundary vegetation (82%). All aphids identified were Amphorophora idaei and Aphis idaei. BRNV and/or RLMV was detected in 27% of the aphid samples. Notably, BRNV was detected in 30% of A. idaei samples, a species not known as a BRNV vector. In subsequent transmission experiments we found that although A. idaei can acquire BRNV within one hour, it did not transmit the virus to healthy raspberry plants. In contrast, Am. idaei, a known BRNV vector, was able to acquire the virus within one minute and transmit it within one hour of inoculation. Our study will improve the identification and management of BRNV.

Název v anglickém jazyce

Incidence of aphid-transmitted viruses in raspberry and raspberry aphids in Norway and experiments on aphid transmission of black raspberry necrosis virus

Popis výsledku anglicky

Raspberry (Rubus idaeus L.) is susceptible to aphid-borne viruses. We studied the incidence of four of them black raspberry necrosis virus (BRNV), raspberry leaf mottle virus (RLMV), raspberry vein chlorosis virus (RVCV), and Rubus yellow net virus (RYNV) in raspberry plants and aphids in and around Norwegian raspberry crops for three years (2019, 2021, and 2022). Most of the samples were from symptomatic plants. Applying RT-PCR, 274 leaf samples and 107 aphid samples were analyzed. All four viruses were found, but BRNV dominated: it was detected in 93% of the 178 leaf samples with virus and was the only virus that occurred more frequently as a single infection than in co-infections with the other viruses. The old cv. Veten had the highest virus incidence (97%) among the sampled plants, followed by uncultivated raspberry in the boundary vegetation (82%). All aphids identified were Amphorophora idaei and Aphis idaei. BRNV and/or RLMV was detected in 27% of the aphid samples. Notably, BRNV was detected in 30% of A. idaei samples, a species not known as a BRNV vector. In subsequent transmission experiments we found that although A. idaei can acquire BRNV within one hour, it did not transmit the virus to healthy raspberry plants. In contrast, Am. idaei, a known BRNV vector, was able to acquire the virus within one minute and transmit it within one hour of inoculation. Our study will improve the identification and management of BRNV.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

<a href="/cs/project/TO01000295" target="_blank" >TO01000295: Zdravé ovoce v měnících se klimatických podmínkách: vývoj nových biotechnologických postupů diagnostiky virů, studium vektorů, ozdravování a bezpečného uchovávání jahodníku a maliníku</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

1664-462X

Svazek periodika

15

Číslo periodika v rámci svazku

November 2024

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

1441145

Kód UT WoS článku

001356566300001

EID výsledku v databázi Scopus

2-s2.0-85209374082